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32,963 results

Showing: 8741 - 8760 of 32963 results

  3. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  6. gNS2B47NS3 S135A

    Plasmid
    #155317
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155317-plasmid-map-sequence-id-311234

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  9. pQCascade-IS186

    Plasmid
    #140627
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140627-plasmid-map-sequence-id-310685

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  16. pET28-Strep-TEV-Nsp14

    Plasmid
    #154955
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    Strep-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154955-plasmid-map-sequence-id-307850

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  19. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Expression
    Bacterial
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154844-plasmid-map-sequence-id-307642

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  20. pASPIre1

    Plasmid
    #154842
    Purpose
    Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Expression
    Bacterial
    Promoter
    Prha
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154842-plasmid-map-sequence-id-307626

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Showing: 8741 - 8760 of 32963 results