34,239 results

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MBP-RGG-SmBit-RGG

Plasmid

#176644

Purpose

SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Publication

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-SmBit-RGG

Expression

Bacterial

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMb2Cas12a

Plasmid

#174676

Purpose

Expresses 6xHis-MBP tagged Mb2Cas12a

Depositor

Piyush Jain

Publication

Nguyen et al medRxiv 2021.07.21

Insert

huMb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165489

Purpose

Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Publication

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

Cdna expression, xenopus oocyte

Tags

Venus

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

Plasmid

#176589

Purpose

Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Chesapeake Bay) locus

Expression

Bacterial

Mutation

Truncated CRISPR array to two direct repeats, rep…

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_minus_cer-region

Plasmid

#175297

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Type

Empty backbone

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Publication

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PTBP1-Halo

Plasmid

#175302

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PTBP1 (PTBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPC-Halo

Plasmid

#175306

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPC (HNRNPC Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Publication

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Publication

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Publication

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (DNM1L Human)

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJH474_pTech_Para-supP

Plasmid

#163718

Purpose

Arabinose-inducible expression of supP

Depositor

Matthew Bennett

Publication

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

supP

Expression

Bacterial

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINO_C1

Plasmid

#171924

Purpose

Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Publication

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C1

Tags

His6

Expression

Bacterial

Available since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Publication

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Publication

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC-C

Plasmid

#174580

Purpose

pOPIN-GG expression vector compatible with C-terminal tagging, carbenicillin resistant

Depositor

Mark Banfield

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Publication

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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