33,526 results
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Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-EmGFP-15b
Plasmid#138280PurposeExpress tTALE-EmGFPDepositorInsert7-mer tTALE-EmGFP
UseTALENTags6x His-tagExpressionBacterialPromoterT7 promoterAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
rpoS200-RFP
Plasmid#63840PurposeThis plasmid is designed as a bacterial stationary phase sensorDepositorInsertrpoS, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Klf4
Plasmid#136613PurposeDox-inducible lentiviral vector expressing mouse Klf4DepositorAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OS
Plasmid#136576PurposeDox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4
Plasmid#136611PurposeDox-inducible lentiviral vector expressing mouse Oct4DepositorInsertOct4 (Pou5f1 Mouse)
UseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHtrA-TEV-His12
Plasmid#137036Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66500.2 (BB_0104 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TEV-12His
Plasmid#137032Purposeempty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)DepositorTypeEmpty backboneTagsHis12 (TEV protease cleavable)ExpressionBacterialPromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_mut_36
Plasmid#139466PurposeT7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36DepositorInsertTarget_mut36
ExpressionBacterialAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFG036
Plasmid#137996PurposecI repressor expression plasmidDepositorInsertcI
UseSynthetic BiologyExpressionBacterialMutationThermosensitiveAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDK
Plasmid#135190PurposeIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selectionDepositorInsertKanR
UseSynthetic BiologyExpressionBacterialPromotercatAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiMPla_N + pSiMPla_C
Plasmid#134314PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertampR_gp41-1 N-intein + ampR_gp41-1 C-intein
ExpressionBacterialAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlh_N + pSiMPlh_C
Plasmid#134316PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInserthygR_gp41-1 N-intein + hygR_gp41-1 C-intein
ExpressionBacterialMutationP254Q in hygR_gp41-1 N-inteinAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP2
Plasmid#129505PurposeExpresses AvicFP2 constitutively in E. coli (most strains)DepositorInsertAvicFP2
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP4
Plasmid#129507PurposeExpresses AvicFP4 constitutively in E. coli (most strains)DepositorInsertAvicFP4
ExpressionBacterialMutationOne of two variants of AvicFP4 tested with essent…Promotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7004
Plasmid#136943PurposeMalachite green aptamer, T7 promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyExpressionBacterialPromoterpT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7005
Plasmid#136944PurposeMalachite green aptamer with stability hairpin, T7 promoterDepositorInsertMalachite green aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRExpressionBacterialMutationnoneAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only