30,880 results

1. pLacI241-Wasabi

   Plasmid

   #86102

   Purpose

   mWasabi reporter gene gated by temperature-sensitive LacI A241T

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   LacIts
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A241T

   Promoter

   T7A1O4/O3 and pLacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PARD3_PDZ_3

   Plasmid

   #103959

   Purpose

   Protein expression and purification of PAR3 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PARD3 (PARD3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2TK cyclin T1 (1-300) (P#432)

   Plasmid

   #14608

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (1-300) (CCNT1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-300

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. p109.1

   Plasmid

   #44518

   Depositor

   Michael Grunstein

   Article

   Hecht et al Cell. 1995 Feb 24;80(4):583-92.

   Insert

   Histone 4
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains full length (aa 1-102)

   Available Since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

5. BPTF

   Plasmid

   #39111

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BPTF (BPTF Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET28-HADH2

   Plasmid

   #67863

   Purpose

   bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   HADH2 (HSD17B10 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet1-His-TEV-OTC_Ub

   Plasmid

   #172134

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF1C-A-RFP

   Plasmid

   #172431

   Purpose

   StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pETM33_N_NTD

    Plasmid

    #156483

    Purpose

    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    N_NTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. polyPRM5

    Plasmid

    #175232

    Purpose

    Bacterial expression and purification, mixed with polySH3 to form condensates

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    ABL1 (ABL1 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST cdc25c

    Plasmid

    #10969

    Depositor

    Toren Finkel

    Article

    Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

    Insert

    cdc25c (CDC25C Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pFVTd5

    Plasmid

    #167520

    Purpose

    Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    VanRAM-tdKatushka2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PVanCC

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSV272-PfMBP-HypDAE

    Plasmid

    #110296

    Purpose

    Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    t-4-Hydroxy-L-proline Dehydratase Activating Enzyme
    

    Tags

    His6 and Maltose Binding Protein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid

    #75355

    Purpose

    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

    Depositor

    Kalle Gehring

    Insert

    Sacsin Xenopus (Silurana) tropicalis 87-1376
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FGFR1_HUMAN_D0

    Plasmid

    #79719

    Purpose

    This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FGFR1 (FGFR1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. SIRT5H158Y-pET15b

    Plasmid

    #81032

    Purpose

    Bacterial expression of Human catalytic mutant SIRT5 protein

    Depositor

    David Lombard

    Article

    Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

    Insert

    Sirt5 (SIRT5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    H158Y catalytic mutant

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE-Tn7-131

    Plasmid

    #118549

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. human p53-Δ(325-355)

    Plasmid

    #24871

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deletion of aa 325-355

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only