30,256 results

1. pET28a(TIGIT)

   Plasmid

   #177902

   Purpose

   Bacterial expression of his10-tagged human TIGIT Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   TIGIT (aa 163-244 only) (TIGIT Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJH021_SC101_pLAC_Cas12c2_Kan

   Plasmid

   #183072

   Purpose

   Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c2x-MT1

   Plasmid

   #32101

   Depositor

   Chris Mercogliano, Cristina Risco Ortiz, David DeRosier

   Article

   Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

   Insert

   Metallothionein (Mt1 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   P-lac

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSEVA221MBPc-AID-T7RNAP

   Plasmid

   #167975

   Purpose

   Expresses fusion protein MBPc-AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   MBPc-AID-T7RNAP
   

   Use

   Tags

   MBPc and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. peSCBE3-NG-HF1

   Plasmid

   #218164

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUG-124

   Plasmid

   #104635

   Purpose

   Level 2 construct

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
   

   Use

   Tags

   HIS-Ubq, HA-E2, GST-E3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a His6Flag2AUF1(p37)

   Plasmid

   #135939

   Purpose

   Expresses AUF1p37 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP42x-2A(sfGFP)

   Plasmid

   #126986

   Purpose

   Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

   Plasmid

   #40731

   Depositor

   Daniel Gerlich

   Article

   Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

   Insert

   Aurora B FRET-sensor
   

   Use

   Tags

   CyPet and YPet

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBdest-GST-PI3Kc1

    Plasmid

    #187992

    Purpose

    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

    Depositor

    Sascha Martens

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKM2 (3GQY)

    Plasmid

    #25360

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PKM2_1 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pET24a MDHC1_ARATH

    Plasmid

    #204237

    Purpose

    Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC1_ARATH (AT1G04410 Mustard Weed)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92228

    Purpose

    Expression of zmADH using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pflaG

    Plasmid

    #45477

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    flaA

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pBZcsx27

    Plasmid

    #89900

    Purpose

    Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx27
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAL3

    Plasmid

    #114673

    Purpose

    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

    Depositor

    William Studier

    Article

    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

    Type

    Empty backbone

    Use

    Tags

    optional Ser-Gly-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PPIC

    Plasmid

    #25606

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PPIC (PPIC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pTD103luxI_sfGFP -R

    Plasmid

    #48887

    Purpose

    Positive feedback plasmid from oscillator without luxR

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    sfGFP and luxI
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-2T_PDZ2

    Plasmid

    #59735

    Purpose

    expresses PDZ2 domain of human ZO-1 in bacteria

    Depositor

    Alan Fanning

    Article

    Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    GST and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pFLxR1

    Plasmid

    #149496

    Purpose

    Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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