31,828 results
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Plasmid#53787Purpose1 kb zebrafish insulin promoter driving eGFPDepositorInsertINS promoter/eGFP/pA
UseZebrafishTagseGFPExpressionBacterialPromoterzebrafish insulin/ZIPAvailable SinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HNRNPC-Halo
Plasmid#175306PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis6-SUMO-g0s2
Plasmid#179293PurposeE. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertG0S2 (G0S2 Human)
TagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterT7Available SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)
Plasmid#181788PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniR-DBCO
Plasmid#181984PurposeUsed to label proteins with the MiniE genetically encoded tagDepositorInsertMiniR-DBCO
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK131
Plasmid#71548PurposeProduces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)DepositorInsertAlanine racemase
ExpressionBacterialPromoterT7Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUMBLA
Plasmid#42420PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPTF
Plasmid#39111PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-cSrc-SH2-AviTag
Plasmid#214230PurposeBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertcSrc kinase SH2 domain (SRC Human)
Tags6xHis, AviTag, and SUMOExpressionBacterialPromoterT7Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_cymR-PCymRC
Plasmid#204071PurposeType 0 promoter partDepositorInsertcymR repressor and PCymRC cumic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in LT)
Plasmid#70033PurposepUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding regionDepositorInsertTSPyV full genome, linearized in Large T-antigen
UseCloning vectorExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMal-E4rGel
Plasmid#51280PurposeExpresses a fibronectin fusion with gelonin targeting EGFR in bacteriaDepositorInsertE4rGel
ExpressionBacterialAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSDH5_G78R
Plasmid#113047PurposeExpression of mutant hSDH5DepositorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOSV802
Plasmid#126595Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321G
Plasmid#98670PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321GDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only