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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Publication

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2∆M

Plasmid

#50489

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Publication

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Delta Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-437

Promoter

pTrc

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2_M

Plasmid

#50490

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Publication

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 438-500

Promoter

pTrc

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb11_3726

Plasmid

#49707

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf11_3726

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGMEH-T38M-750-luxAB-DAS+4

Plasmid

#49520

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

Depositor

Dirk Schnappinger

Publication

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38M-P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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SLIRP.HMG6.wt.fl

Plasmid

#49821

Purpose

expression vector for MBP-SLIRP fusion

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C4

Plasmid

#50356

Purpose

Mouse eIF2C4 cDNA clone

Depositor

Kumiko Ui-Tei

Publication

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C4 (Ago4 Mouse)

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3

Plasmid

#49822

Purpose

for transcribing SRA nucl 419-1356 of NM_001035235 with T7

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pRF-ButR

Plasmid

#49359

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

ButR repressor

Use

Synthetic Biology

Expression

Bacterial

Mutation

E49K

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa03_1198

Plasmid

#49655

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF03_1198

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa42_4454

Plasmid

#49703

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF42_4454

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa28_1088

Plasmid

#49683

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF28_1088

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat5

Plasmid

#45133

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT5

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat2

Plasmid

#45130

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT2

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pLZL2386

Plasmid

#48682

Purpose

pET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)

Depositor

Suzanne Sandmeyer

Publication

Qi et al PLoS One. 2013 May 17;8(5):e63957. doi:

Insert

PFV Integrase

Tags

His

Expression

Bacterial

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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