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pIA033

Plasmid

#200420

Purpose

thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsPAmCherry

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIA034

Plasmid

#200421

Purpose

amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsdronPA

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-Ava3858-3856

Plasmid

#45745

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3856

Expression

Bacterial

Promoter

T7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pMAK462

Plasmid

#140700

Purpose

NBαMs/Rt-IgG1-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs/Rt-IgG1-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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NXR1A

Plasmid

#42430

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NXR1A (NUDT18 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-hGH

Plasmid

#182411

Purpose

Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-hGH (GH1 Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCRISPR_eGFP_191

Plasmid

#111821

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 171-191

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKCiR5

Plasmid

#149462

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-E192C

Plasmid

#180233

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domains (residue…

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTK164

Plasmid

#13533

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTY

Tags

ECFP and Venus

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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L-GARS-2

Plasmid

#166531

Purpose

scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-11

Plasmid

#79926

Purpose

Palm1-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pQO18-TEV

Plasmid

#27399

Depositor

Jonathan Javitch

Article

Zhao et al Nature. 2010 May 13. 465(7295):188-93.

Insert

leuT

Tags

HisX10 and TEV

Expression

Bacterial

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-B MT

Plasmid

#108855

Purpose

Encodes for the fluorescent protein mTurqoise

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

mTurqoise

Expression

Bacterial

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGGB_RT_tdTomato_li

Plasmid

#136964

Purpose

Goldengate/Greengate N-tag module, containing tdTomato fused to a flexible linker

Depositor

Klaus Theres

Insert

tandem Tomato

Expression

Bacterial

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only