30,880 results

1. ZIKV_Sensor_32B_LacZ

   Plasmid

   #75007

   Purpose

   Toehold switch sensor to detect Zika Virus

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_sensor_32B_LacZ
   

   Tags

   LacZ

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1335

   Plasmid

   #119269

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETconNK-TEM1(S70A,D179G)

   Plasmid

   #81166

   Purpose

   pETconNK with TEM1(S70A,D179G) beta-lactamase

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   GAL1 and gal1

   Available Since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. DinoIII

   Plasmid

   #128100

   Purpose

   Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

   Depositor

   Senjie Lin

   Article

   Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

   Insert

   RNA complex & upstream/downstream of LHC
   

   Expression

   Bacterial

   Promoter

   Inserts are potential promoters

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO pTRE

   Plasmid

   #68457

   Purpose

   TOPO construct expressing pTRE for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Tetracycline Response Element Promoter
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET14b PHAS-I

   Plasmid

   #15679

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I (Eif4ebp1 Rat)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pFPV25.1_dGFPmut3-AAV

   Plasmid

   #187377

   Purpose

   rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

   Depositor

   Roi Avraham

   Article

   Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

   Insert

   dGFPmut3_AAV
   

   Tags

   AAV

   Expression

   Bacterial

   Mutation

   C-terminal AAV tail

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSKDuet26

   Plasmid

   #41689

   Depositor

   Hideo Iwai

   Article

   Ellila et al FEBS Lett. 2011 Oct 10.

   Insert

   Tvo VMA intein
   

   Tags

   GB1 and His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM33_WDR5_WD

    Plasmid

    #178491

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    WDR5_WD (WDR5 Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZP10 Snail

    Plasmid

    #16931

    Depositor

    Randall Moon

    Insert

    snail (snai1a Zebrafish)
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

13. Reb_140 aka PAS625

    Plasmid

    #71230

    Purpose

    Reb_1 with RebA(S88P)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA(S88P)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pHlyIIR-H1

    Plasmid

    #74681

    Purpose

    HlyIIR-H1 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSH1-HlyIIR-PHlyIIR-YFP
    

    Expression

    Bacterial

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29b-EPN-01*

    Plasmid

    #80423

    Purpose

    For expression of EPN-01* in E. coli

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*
    

    Tags

    Myc

    Expression

    Bacterial

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pSR357.10

    Plasmid

    #125106

    Purpose

    Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-mEB3-mCherry-6xHis

    Plasmid

    #130985

    Purpose

    Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB3-mCherry-6xHis (Mapre3 Mouse)
    

    Tags

    mCherry-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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