32,684 results
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Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBC13-PGEX4T
Plasmid#25464DepositorAvailable SinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG-NHERF2
Plasmid#28292DepositorAvailable SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22B_PcrA_2Bchimera
Plasmid#102998PurposeExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrADepositorInsertPcrA_2Bchimera
ExpressionBacterialMutationBacilllus stearothermophilus PcrA in which the 2B…PromoterT7Available SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)
Plasmid#136543PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneTagsANXA2 (23-339) and His-tagExpressionBacterialAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP1-kanMX6
Plasmid#133551PurposeYeast fluorescent (RFP) vector with PTEF promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Human ABeta-attR5
Plasmid#160436PurposeEntry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Human)
ExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-YAP-deltaC
Plasmid#21126DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
TagsEGFP-C3ExpressionBacterial and MammalianMutation5 Carboxy-terminal amino acids -FLTWL are deletedAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig1
Plasmid#64841PurposeA lentiviral vector for the expression of mouse Olig1 under doxycyline controlDepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
NEK2
Plasmid#39163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
Tags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD33MevT
Plasmid#17814DepositorInsertMevT
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
Reb_221 aka PAS628
Plasmid#71233PurposeReb_1 with RebA(G90A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, G90A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPro24(s)-gfp
Plasmid#17813DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialMutationD234N compared to AF007834Available SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only