30,810 results

1. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

   Plasmid

   #222989

   Purpose

   Bacterial expression of iGeoCas9(G) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(G)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGex-6p2_FANCM

   Plasmid

   #221379

   Purpose

   Expresses FANCM in bacterial cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Fanconi anemia group M protein (FANCM Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQTEV-MAP1LC3A

   Plasmid

   #34824

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBbA8c-RFP

   Plasmid

   #35272

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BAD

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. UGDH

   Plasmid

   #38900

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   UGDH (UGDH Human)
   

   Tags

   Strep + SET1

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLitR-L1

   Plasmid

   #74683

   Purpose

   LitR-L1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSL1-LitR-PLitR-YFP
   

   Expression

   Bacterial

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CoV-2-NSP3-c001

   Plasmid

   #173083

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E1084-E1192, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET151-Vps4(101-437)-Hcp1

   Plasmid

   #87737

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7-U4

   Plasmid

   #111336

   Purpose

   for in vitro snRNA transcription

   Depositor

   Christine Guthrie

   Article

   Ghetti et al RNA. 1995 Apr;1(2):132-45.

   Insert

   snR14
   

   Expression

   Bacterial

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCJH059_proD_Cas12c_DualRNA-GFP_CAM

    Plasmid

    #183076

    Purpose

    Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and pre-crRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

    Plasmid

    #183070

    Purpose

    Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA targeting GFP
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pelB-MBP-P13-ss

    Plasmid

    #137065

    Purpose

    E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P13: lacks the P13 signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBMTBX-1

    Plasmid

    #26072

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. His6x-EX-HA-FRB-MBD_pYFJ16

    Plasmid

    #120917

    Purpose

    Expresses His6-EX-HA-FRB-MBD in bacteria for protein purification

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    His6-EX-HA-FRB-MBD
    

    Expression

    Bacterial

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MP-K12

    Plasmid

    #69656

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA dinB
    

    Expression

    Bacterial

    Promoter

    E. coli PBAD

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHCKan-INSyibp-Nef

    Plasmid

    #134593

    Purpose

    Low Phosphate inducible Nef

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    HIV Nef protein
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    yibD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJES103

    Plasmid

    #135410

    Purpose

    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

    Depositor

    Miriam Braunstein

    Article

    McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

    Insert

    blaTEM-1
    

    Expression

    Bacterial

    Mutation

    E. coli 'blaTEM-1 with M. tuberculosis mpt63…

    Promoter

    hsp60

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDB021CH(+)

    Plasmid

    #99209

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    PT7 [SD8] gIII, luxAB; ProK pylT
    

    Expression

    Bacterial

    Promoter

    PT7 and PproK

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIA1364

    Plasmid

    #172401

    Purpose

    Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 25 pCOLI_S_NoTag

    Plasmid

    #160261

    Purpose

    Untagged Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge