33,532 results

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pJM919_WT His POLI

Plasmid

#201444

Purpose

Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

6x His

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

lacIq

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Expression

Bacterial

Mutation

abCAS(D537A)

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_9CREN

Plasmid

#204243

Purpose

Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_9CREN

Tags

6X His

Expression

Bacterial

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO2.1

Plasmid

#208017

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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RHOAA-c033

Plasmid

#205116

Purpose

RHOA protein expression

Depositor

Paul Brennan

Insert

RHOA (RHOA Human)

Tags

6His-TEV

Expression

Bacterial

Mutation

WT

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R124A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Expression

Bacterial

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCFT7.2

Plasmid

#208808

Purpose

Expresses the fluorescent protein FAST

Depositor

François-Xavier Campbell-Valois

Publication

Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

Insert

FAST

Expression

Bacterial

Promoter

tac

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISTfu1_reRNA

Plasmid

#205355

Purpose

Bacterial expression of ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISTfu1 TnpB

Expression

Bacterial

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-mOct4 Full length(FL)

Plasmid

#206394

Purpose

Expresses Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Insert

Oct4 (Pou5f1 Mouse)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest-loxP-sfGFP

Plasmid

#202715

Purpose

Empty expression vector containing Cre recombinase reporter.

Depositor

Mary Dunlop

Publication

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Pw7-loxP-term-loxP-sfGFP

Use

Cre/Lox

Expression

Bacterial

Promoter

constitutive

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Annexin A7-TEV-TwinStrep

Plasmid

#198635

Purpose

Annexin A7 fibrillization and lipid binding

Depositor

Lalit Deshmukh

Publication

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 (ANXA7 Human)

Tags

TEV-GS-Dual Strep

Expression

Bacterial

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaSpCas9-NLS-6xHis

Plasmid

#207386

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Publication

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Tags

6xHis

Expression

Bacterial

Available since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pVSL10

Plasmid

#201182

Purpose

Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

Depositor

Jack Szostak

Publication

Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

Insert

Bst polymerase large fragment (BF) F710Y/D598A

Tags

HRV 3C protease cleavage site and His6

Expression

Bacterial

Mutation

F710Y D598A

Promoter

T5-lacO

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Publication

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MPRA_minCMV+GFP

Plasmid

#204866

Purpose

Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

Depositor

Philipp Maass

Publication

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Insert

minCMV:GFP

Expression

Bacterial and Mammalian

Promoter

minimal CMV

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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