34,239 results
-
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CBEv2
Plasmid#221139PurposeCoxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).DepositorInsert3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
UseCRISPRTags3xFLAGExpressionBacterialPromoterlacIq-PtacAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p
Plasmid#202484PurposeExpresses sulfhydryl oxidase (Erv1p) constitutivelyDepositorAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-MBPx-MBPx-SpyTag003
Plasmid#216291PurposeExpresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.DepositorInsertMBPx-MBPx-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM420 GFP-titin-ssrA substrate
Plasmid#226362PurposeExpression of GFP-titin-ssrA substrate for 26S proteasomeDepositorInsertGFP-titin substrate
TagsHis tagExpressionBacterialPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
TagsHis6x tagExpressionBacterialPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
ExpressionBacterialAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM4
Plasmid#225291PurposeE. coli-Campylobacter shuttle vector; TetRDepositorInsertTet(O) and its putative promoter
ExpressionBacterialAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
Tags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOSV00170
Plasmid#222934PurposeConstitutive GFP expression for Bacillus subtilisDepositorInsertGFP(Sp)
UseSynthetic BiologyExpressionBacterialPromoterPhyperspankAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-EYFP
Plasmid#218952PurposeExpresses proinsulin fused to EYFP in bacteriaDepositorInsertproinsulin-EYFP
TagsproinsulinExpressionBacterialPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-mGold
Plasmid#218959PurposeExpresses Hisx6-Tagged mGold in bacteriaDepositorInsertmGold
Tags6X-HisExpressionBacterialPromoterT5Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rab5C(S30C) CL
Plasmid#224273PurposeProtein purification of Rab5C (S30C)DepositorAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx RhoA(G14C) CL
Plasmid#224271PurposeProtein purification of RhoA (G14C)DepositorAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rheb(R15C) CL
Plasmid#224266PurposeProtein purification of Rheb (R15C)DepositorAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx RalA(G23C) CL
Plasmid#224263PurposeProtein purification of RalA (G23C)DepositorAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only