32,684 results
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Plasmid#87689PurposeExpress PhoCl-MBP in E.coliDepositorInsertPhoCl fused to Maltose-Binding Protein
ExpressionBacterialAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP2822
Plasmid#160670PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
137 pGEX2TK RBAP2 HA
Plasmid#11174DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
ExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP28-1A(sfGFP)
Plasmid#126976PurposeLevel 1 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBL066c
Plasmid#227937PurposeExpress Aβ42-GFPDepositorInsertAβ42
TagsGFP (F64L, S65T)ExpressionBacterialMutationF64L and S65T in GFPAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB_syb25
Plasmid#197992PurposeExpressed sybody 25 as a C-terminal myc-His-tagged constructDepositorInsertSybody 25
TagsMyc-HisExpressionBacterialPromoterArabinoseAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP973-1
Plasmid#99496PurposeTEV::linker::meGFP:3XFlagDepositorInsertTEV::linker::meGFP::3XFlag
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCTD
Plasmid#157871Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domainDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SpSuc1
Plasmid#162558PurposeExpresses GST-tagged S.pombe Suc1 in E.coliDepositorInsertSchizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
ExpressionBacterialAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
TagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…Available SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
BASIC_3_Kan
Plasmid#68137Purposestorage vector for BASIC part: KanDepositorInsertKanamycin
ExpressionBacterialAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P1
Plasmid#27366DepositorInsertPmyc1tetO
UseGateway CloningExpressionBacterialMutationN/AAvailable SinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWA PAS-E BAD K-GAFm
Plasmid#39866DepositorInsertsiRFP protein PAS domain
iRFP protein GAF domain (mutated)
TagsE coil and K coilExpressionBacterialMutationF165Y, D232Y, W308RPromoterarabinose inducible and rhamnose inducibleAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_NTD
Plasmid#156483PurposeBacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tagDepositorInsertN_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationS135APromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2B-57
Plasmid#177927PurposeExpresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)DepositorArticleInsertEGFP
ExpressionBacterial and YeastPromoterTET3 (contains three rtTA binding sites)Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
TagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
Tagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only