33,532 results
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Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-cyScarlet
Plasmid#191260PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarletDepositorInsertcyScarlet
TagsHis tagExpressionBacterialAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero45
Plasmid#187931PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero45DepositorAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Human, Synthetic)
TagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorTagsGST-mCherryExpressionBacterialAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
P70a-deGFP-sc101-kan
Plasmid#184841PurposeExpression of deGFPDepositorInsertgreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterpromoter OR2-OR1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-sfGFP
Plasmid#189675Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mEos3.2
Plasmid#189720Purposetitratable expression of mEos3.2 in bacteriaDepositorInsertmEos3.2
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SNAP-23
Plasmid#186686PurposeExpresses SNAP-23 in E. ColiDepositorInsertSNAP23
TagsGST and thrombin siteExpressionBacterialPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
TagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-162
Plasmid#118543PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-His6-Pif6-sfGFP-ssrA
Plasmid#140891PurposeExpression of PIF-sfGFP-ssrA in E. coliDepositorInsertPIF-sfGFP-ssrA
ExpressionBacterialPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.134
Plasmid#187219PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectivelyDepositorInsertA: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseSynthetic BiologyExpressionBacterialPromoterA: pTet*; B: pBetIAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.419
Plasmid#187208PurposeExpresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2DepositorInsertsRetron Eco4 ncRNA
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcAvs4
Plasmid#188794PurposeAvs4 from Escherichia coli NCTC11132DepositorInsertEcAvs4
ExpressionBacterialAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only