31,828 results
-
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT049A
Plasmid#159410PurposeConstitutive murine mature BDNF expression, controlled under E.coli tet promoterDepositorInsertpr.tet-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertet promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
TagsECFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHOB
Plasmid#38801PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (b')
Plasmid#99837PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (b')
TagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3Available SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pThuS
Plasmid#171674PurposeThuS expression in E. coliDepositorInsertGlycosyltransferase ThuS
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5084
Plasmid#168179Purposebacteria reporterDepositorInsertPEM7::dsred PmsrA::sfgfp
ExpressionBacterialMutationnoneAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221MBPc-AID-T7RNAP
Plasmid#167975PurposeExpresses fusion protein MBPc-AID-T7RNAP in E. coliDepositorInsertMBPc-AID-T7RNAP
TagsMBPc and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3a-DJ1
Plasmid#51489PurposeInducible expression of human DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHsSR beta deltaN Flag
Plasmid#13731DepositorInsertSignal recognition particle receptor β subunit (SRPRB Human)
Tags6xHis and FLAGExpressionBacterialMutationexpresses residues 59-271 of Homo sapiens SRβ wit…Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-msGFP2 (C48S C70S)
Plasmid#218956PurposeExpresses proinsulin fused to msGFP2 (C48S C70S) in bacteriaDepositorInsertproinsulin-msGFP2 C48S C70S
TagsproinsulinExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b_NOSER
Plasmid#124244PurposeBacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UGDH
Plasmid#38900PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only