33,537 results
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Plasmid#125835PurposeNegative selection plasmid for evolution of an amber stop codon suppression tRNADepositorInsertACP-2xAmb-pheS
UseSynthetic BiologyExpressionBacterialPromoterpr.trpL*Available sinceMay 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRBV5-1
Plasmid#123414Purposeconstruction of TCRDepositorAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWP11-gfp2
Plasmid#123152PurposepSB1K3 carrying Pecf11-30gfp-tDepositorInsertPecf11-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNG-mTq2
Plasmid#124217PurposeCytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2DepositorInsertmNG-mTq2
ExpressionBacterialAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
ExpressionBacterialPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRAV26-2
Plasmid#123399Purposeconstruction of TCRDepositorAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
Tags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Tre
Plasmid#123129PurposeBacterial expression plasmid for Tre with an N-terminal His-tag.DepositorInsertTre recombinase
Tags6x His tagExpressionBacterialPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_266-341
Plasmid#118821PurposeBacterial expression of hnRNPA2 truncationDepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis and TEVExpressionBacterialMutationcontains amino acids 266-341Available sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_FnCpf1_MBP_protein_expression
Plasmid#120801PurposeProtein expression plasmid for 2xNLS FnCpf1 in pET-21a backboneDepositorInsert2xNLS_FnCpf1
UseCRISPRTagsMBP and NLSExpressionBacterialPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH03C_32
Plasmid#120897PurposeFunctional test of ECF sigma factors and their cognate promotersDepositorInsertECF32_1122
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH04_32_sRNA-A_Sp_coD
Plasmid#120900Purposethe component plasmid of a regulated deviceDepositorInsertspECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (d')
Plasmid#99838PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (d')
TagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
5W9S (CXXC5)
Plasmid#101266PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceApril 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEW65: pQE-81L H3(K79M)
Plasmid#124100PurposeH3K79M expression plasmid for norleucine supplementationDepositorAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3035
Plasmid#122543PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2DepositorAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only