31,841 results
-
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
ExpressionBacterialAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRCPam
Plasmid#195861PurposeEncodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.DepositorInsertAmber Chromogenic Protein on pRCPam plasmid
ExpressionBacterialMutationGln62XAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
ExpressionBacterialAvailable SinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xMyc
Plasmid#129569PurposeEmpty backbone for Myc TagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
SRpHi1
Plasmid#102541PurposeBacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EYFP
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
C2-77 pComb3xss anti-cFos nanobody
Plasmid#145837PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-77 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA4-lenti
Plasmid#115663PurposeExpresses AcrVA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA4
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30 GLTP
Plasmid#170733PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
NM-01
Plasmid#69799PurposeBacterial expression of Cas9 with His, MBP, and Strep tagsDepositorInsertCas9
TagsHIS, MBP, and StrepIIExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …Available SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TurboRFP
Plasmid#103996PurposePlasmid encoding TurboRFPDepositorInsertTurboRFP
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, T68A, L79F, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRU1105
Plasmid#14469DepositorInsertDsRedT3
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
Chx10 3kb AP (CC#318)
Plasmid#15205DepositorInsertChx10 3kb AP reporter (Vsx2 Mouse)
ExpressionBacterialAvailable SinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBLxR5
Plasmid#167504PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxA
Plasmid#188305PurposeExpression of LuxA via a T7 promoter in TXTLDepositorInsertluxA
UseLuciferase and Synthetic BiologyExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
Tags6xHis and FlagExpressionBacterialPromoterT7Available SinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILxR5
Plasmid#149497PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only