32,967 results
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Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
ExpressionBacterialAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
NUDT22 (NUDT22A-c001)
Plasmid#98230PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP758-1
Plasmid#99494PurposeTEV::eGFP::3XFlagDepositorInsertTEV::eGFP::3XFlag
ExpressionBacterialAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP693-3
Plasmid#99492PurposenirFP::3xFlagDepositorInsertnirFP::3xFlag
ExpressionBacterialAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAimR
Plasmid#98687PurposeExpresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tagDepositorInsertAimR
TagsHis-tagExpressionBacterialPromoterNativeAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT16
Plasmid#89659Purposeexpression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT16
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT17
Plasmid#89660Purposeexpression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT17
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT21
Plasmid#89662Purposeexpression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT21
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT32
Plasmid#89667Purposeexpression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT32
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB153
Plasmid#92328PurposeExpress mEos3.2 tagged ZapA in E coliDepositorInsertZapA
TagsmEos3.2ExpressionBacterialPromoterT5-lacAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 CCAAA
Plasmid#98402PurposeBacterial expression vector for Trx1 CCAAADepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 SSAAA
Plasmid#98403PurposeBacterial expression vector for Trx1 SSAAADepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2
Plasmid#91873PurposePhoto-activatable/light sensitive Vav2DepositorAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP tdTomato LIC cloning vector (MBP-tdTomato)
Plasmid#86927PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomatoDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
DEL-pHG165a
Plasmid#90064PurposeLLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmidDepositorInsertDEL
ExpressionBacterialPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_E299A
Plasmid#91855PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) E299A
TagsHis6-MBP-TEV siteExpressionBacterialMutationE299AAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only