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pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

Plasmid

#110554

Purpose

miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

araC-ParaBAD

Expression

Bacterial

Promoter

AraC-ParaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-PO-MT-P2A

Plasmid

#214899

Purpose

Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-PO-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBB530

Plasmid

#27391

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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GST-PRMT1

Plasmid

#34699

Depositor

Steven Clarke

Article

Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

Insert

PRMT1 (Prmt1 Rat)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)

Plasmid

#15385

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS123…

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Reb_1 aka PAS619

Plasmid

#71224

Purpose

Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA G53A

Promoter

T7

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pCN34e-kimA-yfp

Plasmid

#227638

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b_OmpAT7Sav

Plasmid

#138589

Purpose

T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

Insert

T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export

Use

Synthetic Biology

Tags

OmpA export signal and T7 tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046828

Plasmid

#145742

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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