32,967 results
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Plasmid#64169Purposeharbours marker genes LEU2, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCOLA-T7-WspR:G249A
Plasmid#79163PurposeExpresses inactive WspR G249A under a T7 promoterDepositorAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKT3Ts-goldyTALEN
Plasmid#80330PurposeUpdated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequenceDepositorTypeEmpty backboneUseTALENExpressionBacterialPromoterT3Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR276
Plasmid#79577PurposeTP901 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR160
Plasmid#79542PurposeOutput plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR239
Plasmid#79576PurposeA118 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-FAK
Plasmid#74478PurposeThis construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.DepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MetNI (Lm)
Plasmid#78316PurposeMethionine ABC TransporterDepositorInsertsMetI
MetN
Tags10x Histidine Tag and 6x Histidine TagExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-HEPN
Plasmid#75296PurposeExpresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 4441-4579
TagsGST-TagExpressionBacterialAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_4
Plasmid#78208PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak bindingDepositorInsertmatB (His6 C106A R286C T303A Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C T303A Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_3
Plasmid#78207PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak bindingDepositorInsertmatB (His6 C106A T167A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A T167A R286C Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJKW 0643
Plasmid#74121PurposePhotinus pyralis luciferin sulfotransferase LST - pHis8-4DepositorInsertPhotinus pyralis firefly luciferin sulfotransferase
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialPromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-SF3B2 wild type aa 401-550 fragment
Plasmid#67615Purposebacterial expression of wild type GST-SF3B2 fragment (401-550)DepositorInsertsplicing factor 3b subunit 2 (SF3B2 Human)
TagsGSTExpressionBacterialMutationfragment of amino acids 401-550PromotertacAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GALTA-c001
Plasmid#79645PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T-1-RIPK3 4KR
Plasmid#78828PurposeTo purify GST-tagged recombinant RIPK3 4KR proteinDepositorInsertRIPK3 (RIPK3 Human)
TagsGSTExpressionBacterialMutationK55R, K89R, K363R, K501RPromotertacAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAM9
Plasmid#78560PurposepBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.DepositorInsertompU
ExpressionBacterialPromoteraraBAD promoterAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9E (XSB526)
Plasmid#78514PurposeConstruct for expressing His10 - SNAPf -9E in E. ColiDepositorInsertSNAPf
TagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9E added to the C-terminal of SNAPfAvailable SinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only