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pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-E2-Crimson

Plasmid

#111258

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINO_C1

Plasmid

#171924

Purpose

Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C1

Tags

His6

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GST-AR-1-566

Plasmid

#104195

Purpose

Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-566 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pOPS0314

Plasmid

#115507

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB pOGG050, T-pharma pOGG003 assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

Plasmid

#30184

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoAp-GRFT

Plasmid

#158747

Purpose

low phosphate phoAp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoAp Griffithsin

Expression

Bacterial

Promoter

phoA

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTYB11-PTBP1-RRM2CCtoSS

Plasmid

#89154

Purpose

recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRU1105

Plasmid

#14469

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

DsRedT3

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12WTC9AAvi

Plasmid

#47925

Purpose

Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis, Avi Tag, and TEV Cleavage

Expression

Bacterial

Mutation

changed Cysteine 9 to Alanine

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pQE60 R196M wgMDH

Plasmid

#204273

Purpose

Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196M

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D193E wgMDH

Plasmid

#204269

Purpose

Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

D193E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA13c-RFP

Plasmid

#126098

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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UBH14

Plasmid

#28158

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

E3 ligase UHRF2 (UHRF2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

SRA Domain (residues 419-648)

Available Since

Feb. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pHisAlix

Plasmid

#42576

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

ALIX (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only