30,744 results

1. Cyan Nano-lantern/pRSETB

   Plasmid

   #67658

   Purpose

   cyan luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Cyan Nano-lantern
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pZH519

   Plasmid

   #102672

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Expression

   Bacterial

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22-HT/prsA

   Plasmid

   #73624

   Purpose

   Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

   Depositor

   T. J. Kappock, James Williamson

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG184

   Plasmid

   #165604

   Purpose

   Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   'CAGCG' PAM replacing the 'CGGCG&#…

   Promoter

   lac

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MT3

   Plasmid

   #105710

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 3

   Depositor

   Artur Krężel

   Article

   Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

   Insert

   Metallothionein 3 (MT3 Human)
   

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MT4_C30Y R31W

   Plasmid

   #105712

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution

   Depositor

   Artur Krężel

   Insert

   Metallothionein 4 (MT4 Human)
   

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   This subisoform has a C30Y and R31W substitution

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Aurein1.2_(pos36GFP)-Cys

   Plasmid

   #71757

   Purpose

   Aurein1.2_+36GFP-Cys expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-Cys
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB.His.MBP.3C NLRP1 CARD WT

   Plasmid

   #164008

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RAC1 (RAC1A-c007)

   Plasmid

   #110257

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RAC1 (RAC1 Human)
   

   Tags

   StrepII-TEV

   Expression

   Bacterial

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX Txk-SH2

    Plasmid

    #46526

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Txk (Txk Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 138-258

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

    Plasmid

    #80911

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Expression

    Bacterial

    Promoter

    P5G6G and PM,5G6G

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2-cryR;dll4CE1-P1Egfp

    Plasmid

    #90146

    Purpose

    contains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    dll4E1P1Egfp (dll4 Zebrafish)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTG-mTDG-K341R

    Plasmid

    #52267

    Purpose

    Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K341R, SUMO acceptor site mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST17-UbE2Q2

    Plasmid

    #15801

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

15. M62 pPET28a-TEV-human beta cat domain N(1-137)

    Plasmid

    #17203

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    amino-terminal residues 1-137

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pTXB3 AurA

    Plasmid

    #68270

    Purpose

    bacterial expression of human AurA

    Depositor

    Stefano Ferrari

    Article

    Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

    Insert

    AurA (AURKA Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    See depositor comment below

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pNIC-HIS6-SUMO1-GG

    Plasmid

    #215799

    Purpose

    Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRSFDuet-1_StefinA

    Plasmid

    #182402

    Purpose

    Bacterial expression of stefinA

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    stefin (CSTA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_KEAP1_KELCH

    Plasmid

    #178496

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KEAP1_KELCH (KEAP1 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-GST-Laforin

    Plasmid

    #84673

    Purpose

    bacterial expression, GST-fusion protein

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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