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32,691 results

Showing: 11161 - 11180 of 32691 results

  8. pMflET-o4

    Plasmid
    #101314
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    ereB resistance gene
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101314-plasmid-map-sequence-id-198386

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  10. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  11. pETM33_Nsp8

    Plasmid
    #156477
    Purpose
    Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156477-plasmid-map-sequence-id-307732

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  13. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  14. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  18. pOGG004

    Plasmid
    #113979
    Purpose
    Vector construction module. Level 1 golden gate cloning site module with LacZ reporter
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Level 1 golden gate cloning site
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113979-plasmid-map-sequence-id-223412

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Showing: 11161 - 11180 of 32691 results