30,743 results

1. GAMT

   Plasmid

   #25494

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   GAMT (GAMT Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. p53-(1-393)-R175H-N268D

   Plasmid

   #35086

   Depositor

   Cheryl Arrowsmith

   Article

   Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1-393 - R175H-N268D

   Promoter

   T7

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pB1H2w5-12-C81S

   Plasmid

   #18043

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   HhaI C81S mutant
   

   Expression

   Bacterial

   Mutation

   Simply used as an insert between Kpn1 and Xba1

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST TopBP1 B (aa 978-1192)

   Plasmid

   #20372

   Depositor

   Aziz Sancar

   Article

   Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

   Insert

   TopBP1 (978-1192) (TOPBP1 Human)
   

   Expression

   Bacterial

   Mutation

   amino acids 978-1192

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. RECQL

   Plasmid

   #38890

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RECQL (RECQL Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Mutation

   S611G

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA2a-RFP

   Plasmid

   #35325

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-PelB-NH10T-AVITAG-GG

   Plasmid

   #215793

   Purpose

   Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pNIC-HIS6-GST-TEV-AVITAG-GG

   Plasmid

   #215809

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pRSET-B-frSkyran-S

   Plasmid

   #182209

   Purpose

   Bacterial expression of frSkylan-S

   Depositor

   Shiro Suetsugu

   Article

   Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

   Insert

   frSkylan-S
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbS15k-RFP

    Plasmid

    #126194

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23100

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD mPlum

    Plasmid

    #91773

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

    Insert

    monomeric Plum
    

    Tags

    no tag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-PP-Spastin(87-616)DeltaExon4

    Plasmid

    #128794

    Purpose

    Bacteiral expression of Spastin M87 varient without exon 4

    Depositor

    Chris Hill

    Article

    Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

    Insert

    Spastin (SPAST Human)
    

    Use

    `

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 87-616 without exon 4.

    Promoter

    T7

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BACH1

    Plasmid

    #38837

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BACH1 (Bach1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Danio rerio cryabb

    Plasmid

    #47665

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha B-crystallin (cryabb Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQZ083

    Plasmid

    #106417

    Purpose

    Express Complexin-1 fragment (1-83) in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Insert

    complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
    

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Fragment 1-83, Codon optimization

    Promoter

    T7

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL

    Plasmid

    #64177

    Purpose

    harbours marker gene LEU2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE150

    Plasmid

    #118500

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(DAP10)

    Plasmid

    #177904

    Purpose

    Bacterial expression of his10-tagged human DAP10 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    DAP10 (aa 70-92 only) (HCST Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA-GFP

    Plasmid

    #133009

    Purpose

    pFA carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PipaH-TetR

    Plasmid

    #97100

    Purpose

    Expresses TetR in the presence of ipgC and mxiE

    Depositor

    Richard Murray

    Insert

    TetR
    

    Expression

    Bacterial

    Promoter

    PipaH

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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