30,738 results

1. pCYP15

   Plasmid

   #212848

   Purpose

   Expresses the Micromonospora cremea LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Promoter

   PcymRC

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCYP03

   Plasmid

   #212836

   Purpose

   Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Promoter

   PcymRC

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHS1068

   Plasmid

   #205962

   Purpose

   NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH proteins and CRISPR array
   

   Expression

   Bacterial

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. aCE120

   Plasmid

   #204491

   Purpose

   Figure 1. reporter circuit

   Depositor

   Ron Weiss

   Article

   Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

   Inserts

   EBFP2
   mKate2
   EYFP
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial and Mammalian

   Promoter

   hEF1a

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBP-C_mScarlet-I

   Plasmid

   #204110

   Purpose

   Type 0 CDS part

   Depositor

   Mikhail Shapiro

   Insert

   mScarlet-I red fluorescent protein
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLRD26

   Plasmid

   #208273

   Purpose

   Production of levopimaradiene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   LPS
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLRD12

   Plasmid

   #208264

   Purpose

   Production of miltiradiene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   cldB
   smKAL
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SmtA Synechococcus elongatus PCC 7942 Metallothionein

   Plasmid

   #200298

   Purpose

   Bacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 Metallothionein

   Depositor

   Artur Krężel

   Article

   Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

   Insert

   Gene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
   

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDSP23

   Plasmid

   #199372

   Purpose

   Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Available Since

   April 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pJMH147

    Plasmid

    #186647

    Purpose

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKP579

    Plasmid

    #159598

    Purpose

    Express Cba and Cbi protein

    Depositor

    Kathleen Postle

    Insert

    cba and cbi
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a - APX

    Plasmid

    #89618

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Ascorbate dependent Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UBQLN2_450-624W

    Plasmid

    #176852

    Purpose

    Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

    Depositor

    Carlos Castañeda

    Article

    Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

    Insert

    UBQLN2_450C_W (UBQLN2 Human)
    

    Expression

    Bacterial

    Mutation

    deletion of residues 1-449 and addition of C-term…

    Promoter

    T7

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSK+ mBMPR-1B DN

    Plasmid

    #49530

    Purpose

    cloning vector for dominant negative mouse BMP receptor 1B

    Depositor

    Lee Niswander, Peter ten Dijke

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (Bmpr1b Mouse)
    

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also L2V

    Promoter

    T3

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. MKK7

    Plasmid

    #29576

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    MKK7 (Map2k7 Mouse)
    

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Starting Met removed

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pST50Trc1-DHFRHIS

    Plasmid

    #63949

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRF-IcaR

    Plasmid

    #49362

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    IcaR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEM-3z/612

    Plasmid

    #26667

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge