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pQCasTns(Vch)-entry(BsaI)

Plasmid

#190273

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)

Expression

Bacterial

Mutation

no

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Expression

Bacterial

Promoter

pPhlF

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1477

Plasmid

#205970

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH all proteins, with His-tagged Cas11

Tags

14xHis on Cas11

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1801

Plasmid

#205966

Purpose

JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1232

Plasmid

#205969

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9 C287A-His

Plasmid

#11830

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytic mutant (CASP9 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C287A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
Z-AIMP1-1

Plasmid

#166560

Purpose

scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EIF4G2_MIF4G_pGEX

Plasmid

#135138

Purpose

Recombinant expression of EIF4G2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

EIF4G2 (EIF4G2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28 His10-FcRɣ

Plasmid

#113021

Purpose

for purification of His10-FcRɣ intracellular domain

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

His10-FcRɣ intracellular domain

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRC1765

Plasmid

#141346

Purpose

Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

Depositor

Rafael Fernandez-Leiro

Article

Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_WDR5_WD

Plasmid

#178491

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

WDR5_WD (WDR5 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP3

Plasmid

#80659

Purpose

Encodes the synthetic coiled coil peptide SYNZIP3

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP3

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-PaODH

Plasmid

#110802

Purpose

Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4835

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaNAS

Plasmid

#110803

Purpose

Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2469 (SAV2469 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-PaNAS

Plasmid

#110801

Purpose

Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4836

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaODH

Plasmid

#110804

Purpose

Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2468 (SAV2468 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Dhh1-EGFP

Plasmid

#201418

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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