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33,539 results

Showing: 11481 - 11500 of 33539 results

  5. pMS07

    Plasmid
    #230070
    Purpose
    Inducible Promoter (rhamnose-induced, BT3763/rhaR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Publication
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    rhamnose-inducible (BT3763)
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    230070-plasmid-map-sequence-id-486619

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  6. pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

    Plasmid
    #244842
    Purpose
    Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus
    Depositor
    Jennifer Doudna
    Publication
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    Flex-Cas12a
    Use
    CRISPR
    Tags
    4xSV40 NLS and 10xHis-CL7-2xSV40 NLS
    Expression
    Bacterial
    Mutation
    G146R/R182V/D535G/S551F/D665N/E795Q
    Promoter
    T7
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244842-plasmid-map-sequence-id-486525

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  8. MB 45t3 thsS(t3)R-sfGFP_mCherry

    Plasmid
    #232470
    Purpose
    Optimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Publication
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232470-plasmid-map-sequence-id-467797

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  9. pNQ078

    Plasmid
    #242083
    Purpose
    Expresses E. coli RNAP (gene inserts: rpoA-rpoB-rpoC-Cter-ybbR--6xHis and rpoZ)
    Depositor
    Olivier Duss
    Publication
    Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:
    Insert
    rpoA-rpoB-rpoC-Cter-ybbR-6xHis and rpoZ
    Tags
    His6
    Expression
    Bacterial
    Mutation
    ybbR-peptide tag inserted between E1377 and G1402…
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242083-plasmid-map-sequence-id-486119

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  13. pLK06Hpp-Fab CR3009

    Plasmid
    #239861
    Purpose
    Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.
    Depositor
    Urpo Lamminmäki
    Publication
    Korkiakoski et al Protein Expr Purif. 2025 Aug 28:106808.
    Inserts
    Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2 (N, N Human)
    GFP with F64L, S65T, Q80R, M153T and V163A mutations
    Tags
    Hexahistidine tag and bacterial alkaline phosphat…
    Expression
    Bacterial
    Mutation
    F64L, S65T, Q80R, M153T and V163A
    Promoter
    Lac promoter
    Available since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239861-plasmid-map-sequence-id-485412

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Showing: 11481 - 11500 of 33539 results