31,182 results
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Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
TagsmCherryExpressionBacterialAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1
Plasmid#48970PurposeExpresses catalytic domain of MALT1 in e.coli AA 329-566DepositorAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExp-mCherry
Plasmid#112569PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and mCherryExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-24
Plasmid#79939PurposePH-Late1-I3-01-mycDepositorInsertEPN-24
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only