30,735 results

1. pRS415-KEX2

   Plasmid

   #10996

   Depositor

   Francisco Malagon

   Insert

   KEX2 (KEX2 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1 pS43 METTL3

   Plasmid

   #200732

   Purpose

   Bacterial expression of GST-Tagged codon optimized human pS43 METTL3

   Depositor

   Claudio Alarcón

   Insert

   pS43 METTL3 (METTL3 Human)
   

   Tags

   GST from backbone plasmid

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli, UAG co…

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJC20cdc8-cerulean3

   Plasmid

   #99358

   Purpose

   Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

   Depositor

   Dan Mulvihill

   Article

   Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Tags

   Cerulean3 and Gly3-His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

   Plasmid

   #48292

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and Thioredoxin

   Expression

   Bacterial

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB2-mRFP1*

   Plasmid

   #104000

   Purpose

   Plasmid encoding mRFP1*

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRFP1*
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGEE NNLAVIKEF...T…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

   Plasmid

   #168135

   Purpose

   Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLacIq-Wasabi

   Plasmid

   #86112

   Purpose

   mWasabi reporter gene driven by LacIq promoter

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Insert

   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   pLacIq

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pChlr-pNEB193

   Plasmid

   #52136

   Purpose

   A backbone plasmid for creation of additional PUF repeat modules

   Depositor

   Huimin Zhao

   Article

   Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E LAP2b

   Plasmid

   #105978

   Purpose

   3' element for Gateway cloning, lamina-associated polypeptide 2

   Depositor

   Caren Norden

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   LAP2b
   

   Expression

   Bacterial

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK295

    Plasmid

    #71561

    Purpose

    Production of Acetobacter aceti 1023 thioredoxin (AaTrx)

    Depositor

    T. J. Kappock

    Article

    Starks et al Protein Sci. 2007 Jan;16(1):92-8.

    Insert

    thioredoxin
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2P0K

    Plasmid

    #51357

    Depositor

    Cheryl Arrowsmith

    Insert

    2P0K
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pJT106b3

    Plasmid

    #31393

    Depositor

    Christopher Voigt

    Article

    Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

    Insert

    red light inverter with lacZ output
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. BBa_J72110-BBa_J72103

    Plasmid

    #40780

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    AraC-pBad + coi + cin + repL + pacA
    

    Use

    Synthetic Biology; Phagemid

    Expression

    Bacterial

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ2048.2

    Plasmid

    #140687

    Purpose

    3-color stripe (different sgRNAs)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    3-color stripe (different sgRNAs)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCellFree_KA2_ZIKV NS4B

    Plasmid

    #145367

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4B
    

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid

    #153442

    Purpose

    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL0373 (His-MBP-TniQ)

    Plasmid

    #130638

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-his-Gluc-iLID

    Plasmid

    #172096

    Purpose

    Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - iLID
    

    Expression

    Bacterial

    Mutation

    Gluc Mutations - M43L, M110L

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-DUET-Prp18

    Plasmid

    #111354

    Purpose

    protein purification

    Depositor

    Christine Guthrie

    Insert

    PRP18
    

    Expression

    Bacterial

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. JBEI-12050

    Plasmid

    #84982

    Purpose

    Isopentenol production

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Insert

    MevTo-T21-MK-aphA
    

    Expression

    Bacterial

    Promoter

    LacUV5

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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