29,981 results

1. PGEX 4T-1-PPARgamma2

   Plasmid

   #78773

   Purpose

   To purify GST-tagged recombinant protein

   Depositor

   Jaewhan Song

   Article

   Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

   Insert

   PPAR_2
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSB4A3-PBAD-phzH

   Plasmid

   #165618

   Purpose

   Arabinose-inducible expression of phzH for conversion of phenazine-1-carboxylic acid to phanzine-1-carboxamide

   Depositor

   Martin Buck

   Insert

   phzH (phzH Pseudomonas aeruginosa)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC57 DYSF, alt exon 1, 5a, 40a

   Plasmid

   #87889

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a, 40a (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3 14 mut B-Myb

   Plasmid

   #25967

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

   Insert

   B-Myb (Mybl2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Changed threonines 267, 408, 443, 447 490, 497, 5…

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

7. GST-Rab8a-QL

   Plasmid

   #86079

   Purpose

   A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOP5MP

   Plasmid

   #112612

   Purpose

   Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His-MBP and Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HOXA1 (murine) HIS-tag pET

   Plasmid

   #8556

   Depositor

   Corey Largman

   Insert

   HOXA1 (Hoxa1 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   clone contains 130 AA including the homeodomain, …

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pMP89b HeEst

    Plasmid

    #141206

    Purpose

    Halomonas elongata esterase with TEV cleaving site after the N-terminal Histag.

    Depositor

    Francesca Paradisi

    Article

    Padrosa et al Catalysts 2019, 9(3), 232

    Insert

    HeE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet1_CoiC_CoiSA

    Plasmid

    #208762

    Purpose

    Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiC
    CoiSA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    single nucleotide insertion (C70) at RBS of CoiC

    Promoter

    T7

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHL 636

    Plasmid

    #53015

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Inserts

    fimE
    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE30-AtAEP2

    Plasmid

    #159529

    Purpose

    Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

    Depositor

    Joshua Mylne

    Article

    Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

    Insert

    Arabidopsis thaliana asparaginyl endopeptidase 2
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE30-CeAEP1

    Plasmid

    #159528

    Purpose

    Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

    Depositor

    Joshua Mylne

    Article

    Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

    Insert

    Canavalia ensiformis asparaginyl endopeptidase 1
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMUT2-pAto-mCherry

    Plasmid

    #192858

    Purpose

    pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Acetoacetate inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF2

    Plasmid

    #42520

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, T3

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAB675

    Plasmid

    #176578

    Purpose

    Expressing three of the five genes of the n-butanol pathway.

    Depositor

    Arpita Bose

    Article

    Bai et al Commun Biol. 2021 Nov 3;4(1):1257. doi:

    Insert

    phaJ, ter, adhE2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUS232

    Plasmid

    #84579

    Purpose

    Shuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge