29,970 results

1. pET151-GST-PP-RPN13 (19-132)

   Plasmid

   #73742

   Purpose

   Expression of human RPN13 PRU domain in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PRU domain only (residues 19-132)

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pBbB2c-GFP

   Plasmid

   #35344

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBXPHM3-PCFT_NB

   Plasmid

   #165415

   Purpose

   Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

   Insert

   GgPCFT_NB (SLC46A1 Llama, Chicken)
   

   Use

   Tags

   His10-MBP-3C protease site

   Expression

   Bacterial

   Mutation

   Promoter

   arabinose

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM_noro_S2_lacZA

   Plasmid

   #118816

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX Vav2-SH2

   Plasmid

   #46529

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 665-774

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-parkin 219-465

   Plasmid

   #45972

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 219-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-APIC-Cas9

   Plasmid

   #121657

   Purpose

   gateway cloning destination vector for expressing enhancer driven Cas9

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYTK017

   Plasmid

   #65124

   Purpose

   Encodes pRPL18B as a Type 2 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   pRPL18B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. miR21mutant-IRE-TREAT

   Plasmid

   #196931

   Purpose

   Mutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. miR21-IRE-TREAT

    Plasmid

    #196930

    Purpose

    mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Let7-IRE-TREAT

    Plasmid

    #196925

    Purpose

    Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-Squ:mCherry

    Plasmid

    #20163

    Depositor

    Eric Wieschaus

    Article

    Martin et al Nature. 2008 Nov 23. ():.

    Insert

    spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMAL-c2x-MT2

    Plasmid

    #32102

    Depositor

    Chris Mercogliano, David DeRosier, Cristina Risco Ortiz

    Article

    Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

    Insert

    MT (2x) (Mt1 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    P-lac

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. eGFP-Silicatein-pET28

    Plasmid

    #198012

    Purpose

    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

    Depositor

    Bryan Berger

    Article

    Vigil et al Materials Advances 2, 2023

    Insert

    eGFP-silicatein
    

    Use

    Tags

    hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAT

    Plasmid

    #112582

    Purpose

    Used for expression of protein in E. coli as non-cleavable N-terminal 6His-tag fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-Klf4

    Plasmid

    #136613

    Purpose

    Dox-inducible lentiviral vector expressing mouse Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6559 pJC25YDJ1 #7

    Plasmid

    #1245

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Ydj1 (YDJ1 Budding Yeast)
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

19. DNMT1

    Plasmid

    #25165

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    dnmt1.0351.0600 (DNMT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pChlr-8CQ

    Plasmid

    #52171

    Purpose

    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed 12N to 12C in module 8

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only