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pNIC-NHSTIIT-AVITAG-GG

Plasmid

#215802

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-CH10-GG

Plasmid

#215807

Purpose

Cloning of target gene with C terminal non cleavable His10 tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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A2C-∆N

Plasmid

#217909

Purpose

Expresses bicone gas vesicles in E. coli

Depositor

Mikhail Shapiro

Publication

Ling et al Adv Mater. 2024 Feb 26:e2307106. doi: 10

Insert

A2C-∆N

Expression

Bacterial

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Publication

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Expression

Bacterial

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Publication

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

Plasmid

#216387

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

Depositor

William Weis

Publication

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_Null

Plasmid

#213111

Purpose

pkk223 backbone plasmid

Depositor

Martin Horvath

Publication

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Type

Empty backbone

Expression

Bacterial

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYH74

Plasmid

#217382

Purpose

pTrc99A-mNeonGreen-mcdB for bacterial expression

Depositor

Anthony Vecchiarelli

Publication

Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

Insert

mcdB

Tags

NeonGreen

Expression

Bacterial

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYH80

Plasmid

#217387

Purpose

Ptrc99A-mCherry-L78-PopTag for bacterial expression

Depositor

Anthony Vecchiarelli

Publication

Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

Insert

PopTag

Tags

mCherry-Linker78

Expression

Bacterial

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-AqpZ-ALFA

Plasmid

#212310

Purpose

Expression of AqpZ with ALFA tag

Depositor

Arthur Laganowsky

Publication

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

Aquaporin Z

Tags

ALFA tag and Strep-Tag II

Expression

Bacterial

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET16_Caspase-4_p10

Plasmid

#214308

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Expression

Bacterial

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Publication

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6-flag

Expression

Bacterial

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Publication

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-Top2A-5X Gly-Venus-T2A-Hygro

Plasmid

#211115

Purpose

For tagging the C-term of Top2A with Venus

Depositor

Job Dekker

Publication

Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

Inserts

5' C-term Top2A homology arm (TOP2A Human)
Venus
T2A
Blastocidin Resistance
5' C-term Top2A homology arm (TOP2A Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

n/a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I

Plasmid

#208183

Purpose

mistranslation GFP and mScarlet-I positive control

Depositor

Tanel Tenson

Publication

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TAA

Plasmid

#208187

Purpose

nonsense mistranslation, TAA stop

Depositor

Tanel Tenson

Publication

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Publication

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7070

Plasmid

#214008

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (ASV)

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-ASV

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8317

Plasmid

#213989

Purpose

PrpsM driven expression of sfGFP

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

sfGFP

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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