30,735 results
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Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro1GFP
Plasmid#82365PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro1GFP
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMal-mRuby3
Plasmid#209348PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagIIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
TagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…Available SinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-4u
Plasmid#13553DepositorInsertFLIPPi T163A
TagsECFP and EYFPExpressionBacterialMutationT163A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
11 pCOLI_A_MBP
Plasmid#160247PurposeN-term MBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
ExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
TagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Btk-SH2
Plasmid#46412DepositorInsertBtk (BTK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 271-385PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046763
Plasmid#145606PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCOR2
Plasmid#40737PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNCOR2 (NCOR2 Human)
Tags6xHis TEVExpressionBacterialMutationResidues 615 to 685 of the SANT2 domain of Nuclea…Available SinceOct. 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET23b-Casp7 C186A-His
Plasmid#11826DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETpRha-alpha-SkTm
Plasmid#72929PurposeAllows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoterDepositorInsertalpha skeletal Tropomyosin
ExpressionBacterialPromoterrhaBAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MACROD1
Plasmid#39041PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
3535 pET15b Bid p22
Plasmid#8784DepositorAvailable SinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
TagsHis6-GST-3CExpressionBacterialPromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB587
Plasmid#22065DepositorTypeEmpty backboneTagsTAPExpressionBacterialAvailable SinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only