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ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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S2-77 pComb3xss anti-cFos nanobody

Plasmid

#145834

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-H12TEV-hEIF5B-1~1220(FL)

Plasmid

#208636

Purpose

for E. coli expression of human eIF5B (ENST00000289371)

Depositor

Quanfu Ma

Insert

human eIF5B (ENST00000289371) (EIF5B Human)

Tags

6xhis

Expression

Bacterial

Mutation

Contain SNP rs1376443, protein sequence not chang…

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCLIP18

Plasmid

#125142

Purpose

General purpose cloning vector

Depositor

Chris Boyd, David Sherratt

Article

Boyd et al Gene. 1995 Feb 3;153(1):57-62.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-Lux-nat

Plasmid

#120323

Purpose

Bacterial luminescence promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

(None)

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2 E147/8Q

Plasmid

#72217

Purpose

bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

E147Q, E148Q and F404L (please see depositor'…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6_rpoA-C

Plasmid

#190966

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Promoter

T5-lac

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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HSPA5A

Plasmid

#42447

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HSPA5A (HSPA5 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pQE80L TriCatcher-RGDSP-MMP

Plasmid

#112633

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP-MMP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFGL820

Plasmid

#58221

Purpose

For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

ILV2

Expression

Bacterial

Promoter

ILV2 native promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-dCas9-BirA*

Plasmid

#159994

Purpose

Bacterial expression of dCas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

dCas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SMYD3

Plasmid

#25318

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SMYD3_26 (SMYD3 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pHH0103_PIK3R2-1/1

Plasmid

#91415

Purpose

Protein expression and purification of human SH3 domain construct PIK3R2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R2-1/1 (PIK3R2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA.008

Plasmid

#22841

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA (with MCS)

Use

Cotton vigs vector

Expression

Bacterial

Mutation

Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

Plasmid

#80911

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6G and PM,5G6G

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-3z/612

Plasmid

#26667

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pHH0103_RPA2_RPA-C

Plasmid

#109795

Purpose

Protein expression and purification of RPA2_RPA-C

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA2_RPA-C (RPA2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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