34,437 results
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Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core
Plasmid#233618PurposeExpression of His-tagged helicase core region of DDX3X in E.coliDepositorAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialPromoterpBADAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Octo_VirD2_NLS-Cas9-6xHis
Plasmid#232296PurposeExpresses Cas9 protein fused with octopine-type VirD2-originating NLSDepositorInsertOcto_VirD2_NLS-Cas9-6xHis
UseCRISPRTags6xHis and Octopine-type VirD2-derived NLSExpressionBacterialPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1-psHaloTag1a
Plasmid#232940PurposePhotoswichable HaloTag: psHaloTag1a variantDepositorInsertphotoswitchable HaloTag 1a variant
TagsHis6x-HRV3CExpressionBacterialMutationE143W, A258W with linkers L1 (FAG) and L2 (P)PromoterT7Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp41
Plasmid#228207PurposeExpression in BL21(DE3) of T4 helicaseDepositorInsert41 (41 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp59
Plasmid#228206PurposeExpression in BL21(DE3) of WT T4 helicase loaderDepositorInsert59 (59 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_6IYE
Plasmid#234237PurposeHeterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coliDepositorInsert6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_9C74
Plasmid#234238PurposeHeterologous protein expression of variant of super folded GFP in Escherichia coliDepositorInsert9C74
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3KB5
Plasmid#234225PurposeHeterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coliDepositorAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2PPN
Plasmid#234190PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coliDepositorAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1UXZ
Plasmid#234166PurposeHeterologous protein expression of cellulase B in Escherichia coliDepositorInsert1UXZ
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
esmGFP-C10-pVTCB052
Plasmid#231303PurposePlasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coliDepositorInsertesmGFP-C10
ExpressionBacterialAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH81 pET21b Rat1-His
Plasmid#231650PurposeBacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tagDepositorAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
ExpressionBacterialAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-RFP amber
Plasmid#229524PurposemCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13DepositorPublicationInsertRFP amber
ExpressionBacterialPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
WNV isolate WN NY 2000-crow3356 NS5 RdRp full length
Plasmid#228662PurposeBacterial expression of the West Nile virus NS5 RdRp protein for biochemical assayDepositorInsertWest nile NS5 RdRp
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease
Plasmid#228644PurposeBacterial expression of the D68 3C proteaseDepositorInsertEnterovirus D68 3C protease
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits