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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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HS-Utrophin-GFP

Plasmid

#105952

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Utrophin (Utrn Mouse)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-IcaR

Plasmid

#49362

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

IcaR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET-CVm-PTEN

Plasmid

#168032

Purpose

Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-PTEN(M265-N329)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMH-02

Plasmid

#158432

Purpose

pBAD33 vector encoding MotA, eGFP-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

eGFP

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSR284

Plasmid

#125093

Purpose

Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-manR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAT937

Plasmid

#68936

Purpose

Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

Int12

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Stag-YCrs5

Plasmid

#215163

Purpose

Encodes a yeast cadmium metallothionein fused with the S-tag at the N-terminus

Depositor

Travis Beddoe, Bhuvana Shanbhag

Insert

Stag-YCrs5

Tags

S-tag

Expression

Bacterial

Promoter

T7 lac

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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mSNX13-PX (558-677)

Plasmid

#119094

Purpose

Bacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mSNX13-PX (558-677)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX14-PX (561-686)

Plasmid

#119095

Purpose

Bacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX14-PX (561-686) (SNX14 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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NUDT4 (NUDT4A-c001)

Plasmid

#98241

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT4A (NUDT4 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX2T E7 Q

Plasmid

#13648

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 Q mutant (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 30-37 changed from DSSEEEDE to QSSQQQQQ.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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TRIM33 (TRIM33B-c100)

Plasmid

#98243

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TRIM33B (TRIM33 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TUBG1

Plasmid

#171967

Purpose

pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG1 (TUBG1 Human)

Tags

GST

Expression

Bacterial

Mutation

R275H (Please see depositor comments)

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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4QQ6

Plasmid

#61946

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SMN1:JMC047-F11:C221089 (SMN1 Human)

Tags

6xHis and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits