30,307 results

1. pEB1-Clover

   Plasmid

   #103991

   Purpose

   Plasmid encoding Clover

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Clover
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. T4 Lysozyme N68C A93C WT*

   Plasmid

   #18375

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N68C A93C WT*

   Promoter

   Available since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pNIC28-BIRC7

   Plasmid

   #196363

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   BIRC7/Livin
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIC28-BIRC3

   Plasmid

   #196366

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   BIRC3/cIAP2
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM5089

   Plasmid

   #85125

   Purpose

   KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

   Depositor

   Susan Golden

   Article

   Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

   Insert

   PkaiB-kaiB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSVD5

   Plasmid

   #18877

   Depositor

   Jay Keasling

   Article

   Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

   Insert

   exopolyphosphatase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-parkin 219-465

   Plasmid

   #45972

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 219-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. E17TAG GFP zeo resistance

   Plasmid

   #69118

   Purpose

   For E17TAG GFP reporter expression in non-zeocin resistant strains

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   E17TAG GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17 to TAG

   Promoter

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMAL-c2x-MT2

    Plasmid

    #32102

    Depositor

    Chris Mercogliano, Cristina Risco Ortiz, David DeRosier

    Article

    Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

    Insert

    MT (2x) (Mt1 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    P-lac

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pHL 636

    Plasmid

    #53015

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Inserts

    fimE
    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p5426

    Plasmid

    #168187

    Purpose

    bacteria reporter

    Depositor

    Michael Hensel

    Article

    Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

    Insert

    PBAD::dsred PtetA::sfgfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQnl_tet

    Plasmid

    #191355

    Purpose

    Clostridium expression vector with TetR-repressed NanoLuc expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PGus2A-TetO2/1

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_UEVLD_UEV

    Plasmid

    #109902

    Purpose

    Protein expression and purification of UEVLD_UEV

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    UEVLD_UEV (UEVLD Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_ARHGEF9-1/1

    Plasmid

    #91417

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF9-1/1 (ARHGEF9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM-3z/607

    Plasmid

    #26660

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pet16b ALIX Bro1 (1-359)

    Plasmid

    #80641

    Purpose

    Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    ALIX Bro1, residues 1-359 (PDCD6IP Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Residues 1-359

    Promoter

    T7

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS101

    Plasmid

    #118280

    Purpose

    Expresses GFPmut2 with low cell-to-cell variation on a low-copy plasmid and tetracycline induction

    Depositor

    Zach Hensel

    Article

    Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSM17

    Plasmid

    #101641

    Purpose

    Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    eqFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU2S-b (colE1 origin)

    Plasmid

    #74092

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge