31,059 results
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Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
TagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Cas12c2-noncoding
Plasmid#120877PurposeExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.DepositorInsertNoncoding sequences for CRISPR-Cas12c2
UseCRISPRExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS21: pHelper(PmcCAST)_entry
Plasmid#168154PurposeInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pKCyGe2
Plasmid#167515PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.DepositorInsertCymRAM-GmR
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 FL
Plasmid#47090DepositorAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-OleD
Plasmid#59372PurposeInducible expression of OleD (DQ195536) under arabinose promoterDepositorInsertOleD-His
ExpressionBacterialPromoterpBADAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD009
Plasmid#116850PurposeExpresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009DepositorInsertsAraC
alpha-HL
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateExpressionBacterial and YeastAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPXc
Plasmid#38328DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
Tags6xHIS, GST, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
RBM15B_3xRRM_pGEX
Plasmid#135125PurposeRecombinant expression of RBM15B with dual-affinity tagDepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD433
Plasmid#63756PurposeDerivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now TcsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP
Plasmid#67743PurposePr promoter expressing deGFPDepositorInsertUTR1-deGFP-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-PrAvailable SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS-mPlum
Plasmid#117396PurposeTn7 tagging vector pTn7xKS with mPlum expression scaffoldDepositorInsertmPlum
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAPDHS
Plasmid#39010PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
11 pCOLI_A_MBP
Plasmid#160247PurposeN-term MBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and MBPExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only