29,920 results

1. p47phox-PX (1-122)

   Plasmid

   #119124

   Purpose

   Bacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   p47phox-PX (1-122)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-EsaR-D91G-EsaI

   Plasmid

   #47662

   Purpose

   esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   esaI-term
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pBabe-PI(WT)-Rac1(Q61L)-mCherry

   Plasmid

   #87553

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Rac1 (RAC1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Q61L

   Promoter

   CMV

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEDL3

   Plasmid

   #25092

   Depositor

   Christopher Voigt

   Article

   Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

   Insert

   Edge detector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS DKK3

   Plasmid

   #19201

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Dickkopf homolog 3 (DKK3 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

6. His-Sin3a-115-212(pL01)

   Plasmid

   #124386

   Purpose

   E.Coli produced SIN3A PAH1 purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   His-Sin3a-115-212 (SIN3A )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMH-HT_ygiF-CHAD

   Plasmid

   #127793

   Purpose

   Expresses the CHAD domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF-CHAD (ygiF Escherichia coli)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 201-433)

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCARSF85

   Plasmid

   #64694

   Purpose

   H6-TF-Smt3-GB1-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, NpuDnaE-N102, Smt3, and TF

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNAS1b split mCherry 14-3-3β

   Plasmid

   #111880

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Use

   Tags

   Split mCherry system: 14-3-3β fused to C-terminal…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. STK10

    Plasmid

    #38830

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    STK10 (stk10 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBS huNFATc4-EE

    Plasmid

    #17869

    Depositor

    Jerry Crabtree

    Article

    Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

    Insert

    NFATc4 (NFATC4 Human)
    

    Use

    Tags

    EE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

12. RRAS

    Plasmid

    #38815

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RRAS (Rras Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET32a-hAREG

    Plasmid

    #199229

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Amphiregulin preproprotein (AREG Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid

    #184840

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and lac promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p_SNIPR_target_GUmut33AtoG

    Plasmid

    #139468

    Purpose

    T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_mutGU33
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/HIP1/ΔC

    Plasmid

    #31250

    Depositor

    Theodora Ross

    Article

    Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

    Insert

    Huntingtin-interacting protein 1 (HIP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deltion of nucleotides 1336-2513 1386bp C to T…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pRF-ButR

    Plasmid

    #49359

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    ButR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E49K

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHPT1A

    Plasmid

    #42360

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHPT1A (PHPT1 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. TDP-43_NTD1-80_E17R

    Plasmid

    #104479

    Purpose

    bacterial expression of TDP-43 NTD E17R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17R

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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