30,695 results

1. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nanog-luciferase targeting construct

   Plasmid

   #26591

   Depositor

   Rudolf Jaenisch

   Article

   Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

   Insert

   Nanog promoter-luciferase-lox-pgk-neomycin-loc
   

   Expression

   Bacterial

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

   Plasmid

   #30185

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11-His6-Pif6-sfGFP-ssrA

   Plasmid

   #140891

   Purpose

   Expression of PIF-sfGFP-ssrA in E. coli

   Depositor

   Neal Devaraj

   Article

   Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   PIF-sfGFP-ssrA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW414hrpL-cIgfp

   Plasmid

   #61439

   Purpose

   pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter

   Depositor

   Martin Buck, Baojun Wang

   Article

   Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

   Insert

   HrpL-rbs34-CI-LVA-t-plam-30gfp-t
   

   Tags

   LVA

   Expression

   Bacterial

   Promoter

   HrpL

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Brg1 Del1 pET28a

   Plasmid

   #122115

   Purpose

   Expression Brg1 amino acids 1-381 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Tags

   His-T7

   Expression

   Bacterial

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExodus

   Plasmid

   #39991

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   CMV, T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TTR C10S S85C K80D

   Plasmid

   #184743

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, K80D

   Promoter

   T7

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-T ZfHoxc11a

    Plasmid

    #27542

    Depositor

    Victoria Prince

    Insert

    Hoxc11a (hoxc11a Zebrafish)
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGEX6P1-DEST-HA

    Plasmid

    #119751

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Tags

    GST and HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV114

    Plasmid

    #63181

    Purpose

    pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Endophilin II Full

    Plasmid

    #47409

    Depositor

    Peter McPherson

    Insert

    Endophilin II
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pGBW-m4046901

    Plasmid

    #145725

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. MAP3K3

    Plasmid

    #38937

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAP3K3 (MAPK3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. Lenti-BAT-RED

    Plasmid

    #20674

    Depositor

    Tannishtha Reya

    Insert

    Wnt reporter dsRED
    

    Tags

    NLS

    Expression

    Bacterial

    Available Since

    May 26, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

    Plasmid

    #73300

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Tags

    intein

    Expression

    Bacterial

    Mutation

    N1043S-Q1047E-S1079N-E1083Q

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pETDuet RagA-RagC(S75N)

    Plasmid

    #99645

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S75N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG8Ha-SERBP1(149-400)

    Plasmid

    #176516

    Purpose

    Expresses the human C-terminal SERBP1 149-400 construct.

    Depositor

    David Libich

    Article

    Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

    Insert

    SERBP1 149-400 (SERBP1 Human)
    

    Tags

    His8 tag, linker, TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-148 and 401-408

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FDPS

    Plasmid

    #39131

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FDPS (FDPS Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only