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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-19b_MMLV-RT

Plasmid

#153312

Purpose

Bacterial expression of MMLV reverse transcriptase

Depositor

Drew Endy, Philippa Marrack

Insert

MMLV Reverse Transcriptase

Tags

10x His

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-MCM3/5

Plasmid

#116950

Purpose

Expresses human MCM3 and human MCM5

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM5 (MCM5 Human)
human MCM3 (MCM3 Human)

Tags

His6

Expression

Bacterial

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

Plasmid

#29722

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p2H12Matry-blind

Plasmid

#221181

Purpose

cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry(blind)

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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His-ATG3

Plasmid

#202777

Purpose

ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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KAT2A

Plasmid

#38914

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

KAT2A (KAT2A Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pSmartBAC-S

Plasmid

#107268

Purpose

Broad host range bacterial artificial chromosome vector

Depositor

Ron Godiska, Mark Liles

Article

Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

Type

Empty backbone

Expression

Bacterial

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-7560.1

Plasmid

#111406

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 7560 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-7560.1

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJA5 H-2K(b)

Plasmid

#180441

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2K(b)

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBud-EGFP

Plasmid

#23027

Depositor

Andrea LeBlanc

Article

Jodoin et al PLoS One. 2009 . 4(8):e6647.

Insert

EGFP (eGFP )

Expression

Bacterial and Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR L1-13XLexAop2-L4

Plasmid

#41434

Depositor

Steve Stowers

Insert

13XLexAop2

Expression

Bacterial

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pKB237

Plasmid

#170030

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 (CCL19 Synthetic, Escherichia coli, Human)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMC037-HisMS2_PLP_pac

Plasmid

#128233

Purpose

Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1356

Plasmid

#119276

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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