30,736 results

1. pRSFDUET-1_VNp-anti-6xHis mAb

   Plasmid

   #214952

   Purpose

   Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-6xHis mAb heavy chain
   VNp-anti-6xHis mAb light chain
   

   Tags

   VNp6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJL3

   Plasmid

   #199378

   Purpose

   Encodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   jGCaMP7s::SL2::mScarlet-I
   

   Expression

   Bacterial

   Mutation

   no mutations

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET mCherry LIC cloning vector (u-mCherry)

   Plasmid

   #29769

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOSIP-CT (CamR, P21)

   Plasmid

   #45981

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7C-HSPA8

   Plasmid

   #177661

   Purpose

   Recombinant protein synthesis of human HSPA8

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA8 (HSPA8 Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD_FLAG-GFP(Y39TAG)-6His

   Plasmid

   #223510

   Purpose

   Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Insert

   GFP(Y39TAG)
   

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   Y39TAG

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTECH-chAcK3RS(IPYE)

   Plasmid

   #104069

   Purpose

   Expression plasmid encoding chAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chAcK3RS(IPYE); PproK pyl
   

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTXB1-CfaC-Gyra

   Plasmid

   #122950

   Purpose

   Bacterial expression of C-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaC
   

   Expression

   Bacterial

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SBFP2-anti-AlfaTag nanobody

    Plasmid

    #171826

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    SBFP2-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBJ7

    Plasmid

    #167136

    Purpose

    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB2-E2-Crimson

    Plasmid

    #104010

    Purpose

    Plasmid encoding E2-Crimson

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    E2-Crimson
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pNF02-mChartreuse

    Plasmid

    #219397

    Purpose

    Constitutive expression of mChartreuse in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mChartreuse
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b(+)_SNAP-tag2

    Plasmid

    #226512

    Purpose

    Bacterial cell expression of SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    SNAP-tag2
    

    Tags

    His-tag (10x) and Strep-Tag II,

    Expression

    Bacterial

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Impact-T4gp43(exo-)

    Plasmid

    #228202

    Purpose

    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated aspartate 219 to alanine (D219A), elimina…

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLacI

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD103

    Plasmid

    #119887

    Purpose

    Constitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

    Insert

    pConstitutive_mCherry
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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