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pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRAF-MutH

Plasmid

#86609

Purpose

E. coli MutH expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutH

Tags

his-tag and ald-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_PTK2b

Plasmid

#224237

Purpose

Expression of human PTK2b Kinase in E. coli

Depositor

Andrew Mesecar

Insert

ptk2b (PTK2B Human)

Tags

6xHis and 6xHis-TEV

Expression

Bacterial

Promoter

T7 promotor

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

Plasmid

#48287

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and msfGFP

Expression

Bacterial

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pProEx Rheb(R15C) CL

Plasmid

#224266

Purpose

Protein purification of Rheb (R15C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rheb (RHEB Human)

Expression

Bacterial

Mutation

R15C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-EFSep

Plasmid

#34627

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

EF-Sep (tufB E. coli)

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only
pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Expression

Bacterial

Mutation

MphR: N94D

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pEB2_mScarletI_mVenusNB-MrkHR113A

Plasmid

#182292

Purpose

expresses c-di-GMP mutant biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkHR113A

Tags

mVenusNB internal fusion to MrkH with a R113A poi…

Expression

Bacterial

Mutation

changed arginine 113 to alanine, MrkH numbering …

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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