29,996 results

1. pSL1765 (pSPIN-R, R*MmeI)

   Plasmid

   #160734

   Purpose

   Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM157

   Plasmid

   #45863

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Insert

   cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-his-3C_Tau352

   Plasmid

   #100092

   Purpose

   Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

   Depositor

   Nikolai Sluchanko

   Article

   Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

   Insert

   Fetal microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   wild type sequence

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB1

   Plasmid

   #62547

   Purpose

   broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

   Depositor

   Michael Franklin

   Article

   Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

   Insert

   eGFP
   

   Use

   Broad host range plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR0306 CcaCas13b His6-TwinStrep-SUMO

   Plasmid

   #182687

   Purpose

   Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   CcaCas13b
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. M13KO7-ΔPS-ΔgeneIII

   Plasmid

   #134351

   Purpose

   Helper phage for M13 phage production

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Helper phage gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIVM_26

   Plasmid

   #204501

   Purpose

   Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   0

   Promoter

   AmpR

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-MNase

   Plasmid

   #136291

   Purpose

   Expresses Micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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