4,908 results

1. pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

   Plasmid

   #238314

   Purpose

   ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Use

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-536-1107-delta848-918-R547A-H548A-R551A

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBB3-ScVip1-536-1107-R547A-H548A-R551A

   Plasmid

   #238317

   Purpose

   ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Use

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-536-1107-R547A-H548A-R551A

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1pBB3-ScVip1-536-1107-C793A

   Plasmid

   #238324

   Purpose

   1ScVip1-536-1107-C793A protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Use

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-536-1107-C793A

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

   Plasmid

   #238327

   Purpose

   ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Use

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-536-1107-E576A-K623A-Q625S-K627A

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBB3-ScVip1-186-522

   Plasmid

   #238311

   Purpose

   ScVip1-186-522 protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Use

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-186-522

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

   Plasmid

   #240228

   Purpose

   Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

   Plasmid

   #240229

   Purpose

   Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT-sfGFP-TurboID-ER

   Plasmid

   #240230

   Purpose

   Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   BIP-sfGFP-TurboID-KDEL
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT-sfGFP-TurboID

   Plasmid

   #240231

   Purpose

   Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   sfGFP-TurboID
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAS-sfGFP-TurboID-ER

    Plasmid

    #240232

    Purpose

    Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BIP-sfGFP-TurboID-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT-Myc-BirA*G3-ER

    Plasmid

    #240234

    Purpose

    Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BiP-Myc-BirA*G3-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUAS-spatzle-HA

    Plasmid

    #240235

    Purpose

    Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    spatzle (spz Fly)
    

    Use

    Tags

    HA-spGFP11

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUAS-hhN-HA

    Plasmid

    #240236

    Purpose

    Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Use

    Tags

    HA-spGFP11

    Expression

    Insect

    Mutation

    aa 1-257

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAS-CG6867-sfGFP

    Plasmid

    #240237

    Purpose

    Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    CG6867 (CG6867 Fly)
    

    Use

    Tags

    sfGFP

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWal10-roe-sfGFP

    Plasmid

    #240239

    Purpose

    Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT-EGFP-V5

    Plasmid

    #240240

    Purpose

    Expression of EGFP-V5 under control of copper-inducible promoter.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    EGFP-V5
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-GMR-eya(shRNA)_EcoRImut

    Plasmid

    #240222

    Purpose

    pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6:3-tevopreq1

    Plasmid

    #190909

    Purpose

    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6:2-U6:3-tevopreq1

    Plasmid

    #190910

    Purpose

    Expression of nicking sgRNA and epegRNA under control of separate Drosophila U6:2 and U6:3 promoters. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBB3-ScVip1-536-1107-H548A

    Plasmid

    #238319

    Purpose

    ScVip1-536-1107-H548A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-H548A

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge