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pMB.003

Plasmid

#228669

Purpose

Recombination to BV DNA (Tn7)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Tn7L

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.002

Plasmid

#228668

Purpose

Moclo Baculo toolkit level 2 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.001

Plasmid

#228667

Purpose

Moclo Baculo toolkit level 1 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pM.002

Plasmid

#228688

Purpose

C terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Prs-MBP

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pM.001

Plasmid

#228687

Purpose

N terminal MBP affinity tag with PreScission Protease cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

MBP-Prs

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-400)

Plasmid

#228830

Purpose

Insect cell expression of N-terminal portion of BICD2 (aa 1-400)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 1-400

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-710)

Plasmid

#228831

Purpose

Insect cell expression of truncated BICD2 (aa 1-710)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 1-710

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(342-800)

Plasmid

#228832

Purpose

Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 342-800

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L742A

Plasmid

#228839

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L742A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX R139A

Plasmid

#192058

Purpose

Expression vector for SOX (ORF37) R139A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

R139A

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX 318-320A

Plasmid

#192060

Purpose

Expression vector for SOX (ORF37) 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

318-320A

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac WT SOX

Plasmid

#192055

Purpose

Expression vector for WT SOX

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX (ORF37)

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

Plasmid

#222300

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[451-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

Plasmid

#222301

Purpose

Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK3[571-718] (HOOK3 Human)
FTS (AKTIP Human)
FHIP1B (FHIP1B Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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