4,908 results

1. pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

   Plasmid

   #217514

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFB1.HMBP.PrS.EZH2-mt2

   Plasmid

   #220240

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFB1.HMBP.PrS.EZH2-mt1

   Plasmid

   #220239

   Purpose

   Expression of an EZH2 mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   R27A, R31A, F165A

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

   Plasmid

   #217509

   Purpose

   KD Recombinase dependent conditional 1X ALFA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
   

   Use

   Tags

   ALFA tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 1167A

   Plasmid

   #218233

   Purpose

   HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene

   Depositor

   Omar Akbari

   Insert

   putative metabolite transport protein
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MBP-3C-hTspan12LEL(118-218)_pAcGP67a

   Plasmid

   #216383

   Purpose

   Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Tetraspanin-12 (TSPAN12 Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mFzd4CRDL(42-203)-6xHis_pAcGP67a

   Plasmid

   #216379

   Purpose

   Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis and GP64 signal sequence

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. I14-pAc5.1 lambda N HA Cup

   Plasmid

   #206493

   Purpose

   Cup expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Use

   Tags

   lambda N HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. F23-pAc5.1 EGFP Cup

   Plasmid

   #206488

   Purpose

   Cup expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Cup
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. F17-pAc5.1 EGFP Vasa 1-200

    Plasmid

    #206484

    Purpose

    vasa 1-200 expression for Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Vasa 1-200
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDB_052

    Plasmid

    #216082

    Purpose

    for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Hygromycin resistance gene
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRDB_051

    Plasmid

    #216081

    Purpose

    for stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    attL and attR sequences for genome integration by phiC31
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFBOH-LIC_MAGEA3:104-314

    Plasmid

    #210941

    Purpose

    Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    MAGEA3:E104-E314 (MAGEA3 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pFBOH-avi-TEV-LIC_DCAF12:1-453

    Plasmid

    #210940

    Purpose

    Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pFBOH-MHL_EIF2A:1-427

    Plasmid

    #210939

    Purpose

    Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EIF2A:M1-T427 (EIF2A Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFBOH-MHL_UTP15:1-370

    Plasmid

    #210937

    Purpose

    Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    UTP15:M1-H370 (UTP15 Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pFBOH-MHL_TBL2:70-405

    Plasmid

    #210936

    Purpose

    Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    TBL2:E70-H405 (TBL2 Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFBOH-MHL_SHKBP1:182-585

    Plasmid

    #210935

    Purpose

    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SHKBP1:T182-L585 (SHKBP1 Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    Q507L natural variant (VAR_036714)

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge