4,841 results

1. pAc5.1B-lambdaN-HA-DmMextli_AA

   Plasmid

   #148213

   Purpose

   Insect Expression of DmMextli

   Depositor

   Elisa Izaurralde

   Insert

   DmMextli (mxt Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   4 silent mutations compared to the sequence given…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc5.1B-EGFP-DmeIF4A-dsRNAres_AA

   Plasmid

   #148201

   Purpose

   Insect Expression of DmeIF4A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmeIF4A-dsRNAres (eIF-1A Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

   Plasmid

   #147007

   Purpose

   Insect Expression of DmUPF3-K31E-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3-K31E-siRNAres (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and one non silent mutation P305S compar…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

   Plasmid

   #146990

   Purpose

   Insect Expression of DmUPF1_92-1180-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF1_92-1180-siRNAres (Upf1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   1 non silent mutations: G995E compared to the seq…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

   Plasmid

   #146957

   Purpose

   Insect Expression of DmAGO1-K690A

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

   Insert

   DmAGO1-K690A (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations V315A and H…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1C-Fluc-nerfin-HSL_E

   Plasmid

   #146228

   Purpose

   Insect Expression of Nerfin-3UTR

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   Nerfin-3UTR (nerfin-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Nucleotide mutations A>T 4387 and A>T 4955 …

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M

   Plasmid

   #146901

   Purpose

   Insect Expression of DmCup_1-417-Y342AL347A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

   Insert

   DmCup_1-417-Y342AL347A (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmMe31B-mut1_E

   Plasmid

   #146203

   Purpose

   Insect Expression of DmMe31B-mut1

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmMe31B-mut1 (me31B Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. S+Q111R

   Plasmid

   #167173

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+Q111R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. S+N122D

    Plasmid

    #167174

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed N at 111 to D

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. S+Q111R+N122D

    Plasmid

    #167175

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R+N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R and changed N at 122 to D

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. S+10subs

    Plasmid

    #167176

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+10R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed A at 112 to T; E at 116 to D; I at 135 to…

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. S+12subs

    Plasmid

    #167177

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+12R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R; A at 112 to T; E at 116to …

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACEBac1-gamma-tubulin(N229A)-TEV-His6

    Plasmid

    #178077

    Purpose

    Insect cell expression of gamma-tubulin(N229A)-TEV-His6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    gamma-tubulin with an N229A point mutation (TUBG1 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Changed Asparagine 229 to Alanine

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

    Plasmid

    #178074

    Purpose

    Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    MZT2A (MZT2A Human)
    

    Use

    Tags

    PreScission-mEGFP and ZZ-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACEBac1-gamma-tubulin-TEV-His6

    Plasmid

    #178067

    Purpose

    Insect cell expression of gamma-tubulin-TEV-His6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACE-Truncated-Kir3.2-StreptagII

    Plasmid

    #177263

    Purpose

    Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

    Insert

    G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
    

    Use

    Tags

    StreptagII

    Expression

    Insect

    Mutation

    Residues 49-378

    Promoter

    polyhedrin promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SYKA-c014

    Plasmid

    #175488

    Purpose

    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-Zbasic-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOmnibac_ZZ_linker_K490_GFP_SNAP

    Plasmid

    #159721

    Purpose

    Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-490), K490 (KIF5B Human)
    

    Use

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids (1-490)

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOmnibac_ZZ_linker_ybbR_MAP7_N

    Plasmid

    #159728

    Purpose

    Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    MAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)
    

    Use

    Tags

    ZZ affinity tag and ybbR tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLib6.4-Cquin_728

    Plasmid

    #176656

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLib6.4B-Agam_557

    Plasmid

    #176667

    Purpose

    Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLib6.4B-Aaeg_972

    Plasmid

    #176674

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLib6.4-Agam_557

    Plasmid

    #176653

    Purpose

    Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLib6.4B-Aalb_726

    Plasmid

    #176675

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLib6.4B-Aaeg_763

    Plasmid

    #176672

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLib6.4-Aaeg_972

    Plasmid

    #176660

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLib6.4-Cquin_801

    Plasmid

    #176657

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLib6.4B-Cquin_801

    Plasmid

    #176671

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLib6.4B-Cquin_728

    Plasmid

    #176670

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge