4,908 results

1. F19-pAc5.1 EGFP Vasa 463-661

   Plasmid

   #206486

   Purpose

   Vasa 463-661 expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Vasa 463-661
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. F18-pAc5.1 EGFP Vasa 200-462

   Plasmid

   #206485

   Purpose

   Vasa 200-642 expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Vasa 200-462
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. F15-pAc5.1 EGFP Vasa

   Plasmid

   #206483

   Purpose

   Vasa expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Vasa
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. F20-pAc5.1 EGFP Aubergine

   Plasmid

   #206482

   Purpose

   Aubergine expression for Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Aubergine
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDropIn-T2A-ZpGal4DBD

   Plasmid

   #199199

   Purpose

   Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-ZpGal4DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDropIn-T2A-p65Zp

   Plasmid

   #199200

   Purpose

   Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-p65Zp
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p10xUAS-WHaloCaMP1a-EGFP

   Plasmid

   #205314

   Purpose

   Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a-EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBVboostFGII WPRE 3C-CVB3

   Plasmid

   #202077

   Purpose

   Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 3C protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWalium Htt19Q-mCherry

    Plasmid

    #201248

    Purpose

    allows the expression of the insert in Drosophila melanogaster

    Depositor

    Pedro Domingos

    Article

    Branco-Santos et al Hum Mol Genet. 2017 Oct 1;26(19):3763-37

    Insert

    Huntingtin (HTT Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUASt-Xbp1-HA-GFP-3'UTR Spl

    Plasmid

    #201246

    Purpose

    Xbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced form

    Depositor

    Pedro Domingos

    Article

    Cairrao et al Nat Commun. 2022 Mar 24;13(1):1587. doi:

    Insert

    dXbp1 (Xbp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUAST ɑSynWT-EGFP

    Plasmid

    #201249

    Purpose

    allows the expression of the insert in Drosophila melanogaster

    Depositor

    Pedro Domingos

    Article

    Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

    Insert

    ɑ-Synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOET3-C-TEV-Twin-Strep

    Plasmid

    #203324

    Purpose

    Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJFRC7-JEDI-1P

    Plasmid

    #202618

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TEG +Q111T

    Plasmid

    #187435

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed glutamine at 117 to threonine

    Promoter

    PH and p10

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFB-CG-LYCHOS WT-HA-GFP

    Plasmid

    #199687

    Purpose

    Expression of LYCHOS WT in insect cells

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Use

    Tags

    10xHis, EGFP, HA, HRV 3C, and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IR76b-T2A-QF2

    Plasmid

    #162523

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GR3-T2A-QF2

    Plasmid

    #162521

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IR8a-T2A-QF2

    Plasmid

    #162520

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB-CG-LYCHOS FP>IA-HA-GFP

    Plasmid

    #199688

    Purpose

    Expression of LYCHOS FP>IA mutant in insect cells

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Use

    Tags

    10xHis, 6xHis, EGFP, HA, and HRV 3C

    Expression

    Insect

    Mutation

    F43 and P44 mutated to I and A respectively

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge