4,841 results

1. p4xLexAOp-O1

   Plasmid

   #165817

   Purpose

   Omega1 4xLexAOp for assembly of larger LexAOp response elements

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   4xLexAOp-O1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGal4BD 1-147

   Plasmid

   #165819

   Purpose

   1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Gal4BD 1-147
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGAL4AD 768-881

   Plasmid

   #165820

   Purpose

   768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GAL4AD 768-881
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLexA BD

   Plasmid

   #165821

   Purpose

   LexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   LexA BD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQFBD

   Plasmid

   #165823

   Purpose

   QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   QFBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQFAD

   Plasmid

   #165824

   Purpose

   QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   QFAD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGGSx4

   Plasmid

   #165807

   Purpose

   Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GGGSx4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2A

   Plasmid

   #165809

   Purpose

   Viral T2A linker element for assembly of TL3 to TL5 parts

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   T2A
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGGSx4 NT

   Plasmid

   #165810

   Purpose

   Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GGSx4 NT
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Promoterless TP10

    Plasmid

    #165787

    Purpose

    Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Promoterless T10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR20A02

    Plasmid

    #165791

    Purpose

    GB2.0 compatible FlyLight Enhancer element R20A02 in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R20A02
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pR70B04

    Plasmid

    #165792

    Purpose

    GB2.0 compatible FlyLight Enhancer element R70B04 in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R70B04
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pR76H03

    Plasmid

    #165793

    Purpose

    GB2.0 compatible FlyLight Enhancer element R76H03in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R76H03
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCS-TCCC

    Plasmid

    #165774

    Purpose

    Multiple cloning site part for enhancer cloning, requires minimal promoter element

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    MCS-TCCC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pdSCP

    Plasmid

    #165776

    Purpose

    Minimal synthetic Drosophila core promoter, requires enhancer element for full expression

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    dSCP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid

    #172914

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7c-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid

    #172913

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7b-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid

    #171575

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFASTbac-HisGST-PTPIP51(36-470)

    Plasmid

    #170531

    Purpose

    Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
    

    Use

    Tags

    GST tag and His tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACEBac1-Q22YU3-2xStrep

    Plasmid

    #170315

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Mdn1-deltaC

    Plasmid

    #171603

    Purpose

    Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    Mdn1
    

    Use

    Tags

    His6 tag

    Expression

    Insect

    Mutation

    C-terminal deletion; a.a. 1-3911 only

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Mdn1-FL

    Plasmid

    #171602

    Purpose

    Expresses full length S. pombe Mdn1 in insect cells.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    Mdn1
    

    Use

    Tags

    His6 tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

    Plasmid

    #146134

    Purpose

    Insect Expression of DmTral_394-543

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral_394-543 (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations (nucleotides A198C, A387G) a…

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-EGFP-DmBelle_1-298_B

    Plasmid

    #145913

    Purpose

    Insect Expression of DmBelle_1-298

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_1-298 (bel Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    del 158-160 (GGG)

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1C-Fluc-CG7785_A

    Plasmid

    #145863

    Purpose

    Insect Expression of CG7785-3UTR

    Depositor

    Elisa Izaurralde

    Insert

    CG7785-3UTR (CG7785 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    317bp deletion in 3'UTR region (4172-4273bp)…

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBIG1b_nsp7 (SARS-CoV-2)

    Plasmid

    #169178

    Purpose

    Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBIG1c_nsp8 (SARS-CoV-2)

    Plasmid

    #169179

    Purpose

    Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    untagged

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169162

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    6His-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid

    #171574

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge