4,841 results

1. 1026M=U6-3-gRNA#2[Pol-γ35]

   Plasmid

   #159675

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#2[Pol-γ35]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KIF13B

   Plasmid

   #163907

   Purpose

   Full length mouse KIF13B for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Use

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KIF13B 1-584

   Plasmid

   #163908

   Purpose

   Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Use

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Use

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

   Plasmid

   #163927

   Purpose

   Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

   Plasmid

   #163928

   Purpose

   Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

   Plasmid

   #163925

   Purpose

   Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
   

   Use

   Cre/Lox

   Tags

   tagBFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDest-639 (attR4-attR2)

   Plasmid

   #161880

   Purpose

   Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDest-624 (attR4-attR2)

   Plasmid

   #161879

   Purpose

   Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 58 pLIB_SNAP

    Plasmid

    #160307

    Purpose

    N-term 3C cleavable SNAP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    12xHis and SNAP

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc-­His-­zfKitlgb

    Plasmid

    #140293

    Purpose

    Express soluble His-tagged zebrafish Kitlgb in insect cells

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlgb (kitlgb Zebrafish)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc-­His-­zfKitlga

    Plasmid

    #140292

    Purpose

    Express soluble His-tagged zebrafish Kitlga in insect cells

    Depositor

    Petr Bartunek

    Article

    Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

    Insert

    Kitlga (kitlga Zebrafish)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 48 pLIB_C_GST

    Plasmid

    #160273

    Purpose

    C-term uncleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 49 pLIB_3C_C_GST

    Plasmid

    #160274

    Purpose

    C-term 3C cleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 908I

    Plasmid

    #160241

    Purpose

    VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Van Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 907F1

    Plasmid

    #160233

    Purpose

    FlightN-VanR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    VanR
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OA-1045B

    Plasmid

    #125004

    Purpose

    Expresses gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OA-1045D

    Plasmid

    #125006

    Purpose

    Expresses gRNAs targeting hedgehog and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    wg gRNA (wg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    u6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OA-1045E

    Plasmid

    #125007

    Purpose

    Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-986C

    Plasmid

    #125000

    Purpose

    Expresses dCas9-VPR driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VPR
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OA-986D

    Plasmid

    #125001

    Purpose

    Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. OA-986E

    Plasmid

    #125002

    Purpose

    Expresses dCas9-VP64 driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. OA-1045A

    Plasmid

    #125003

    Purpose

    Expresses gRNAs targeting hid and eve

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-attB2-SA(2)-CRY2-mCherry

    Plasmid

    #160441

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC57-attB2-SA(1)-CRY2-mCherry

    Plasmid

    #160440

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC57-attB2-SA(0)-CRY2-mCherry

    Plasmid

    #160439

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. R979-X57-008: Hs.KRAS4b(1-169)

    Plasmid

    #159690

    Purpose

    Baculovirus protein expression of KRAS4b (1-169)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

    Insert

    Hs.KRAS4b(1-169) (KRAS Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Plasmid

    #159557

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    G13D

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

    Plasmid

    #159559

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    Q61R

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Plasmid

    #159555

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    G12D

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge