4,841 results

1. pGS_C_3xFLAG

   Plasmid

   #101653

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, 3xFLAG tag
   

   Use

   Tags

   3xFLAG tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104619

   Purpose

   Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104618

   Purpose

   Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   hsp70-Syn21-FLAG-mCerulean-P10
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

   Plasmid

   #104615

   Purpose

   Expresses GFP upon binding of TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10

   Plasmid

   #104616

   Purpose

   Expresses HA tagged Citrine upon binding of TALE1 to VAS1

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   Syn21-Citrine-HA-P10-MhcSD
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJC-20xUAS-TALE-VP64-P10

   Plasmid

   #104605

   Purpose

   Control plasmid (no TALE array) with P10 terminator

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAFMW-AthHEN1-CM

   Plasmid

   #104981

   Purpose

   Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUAST-Myc-mSesn2 (1186)

   Plasmid

   #66920

   Purpose

   expression of mouse Sesn2 in drosophila

   Depositor

   Jun Hee Lee, Michael Karin

   Article

   Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Insect

   Mutation

   Promoter

   HS promoter

   Available since

   Dec. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCh-623

   Plasmid

   #92266

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   dGW182 6W6A (gw Fly)
   

   Use

   Tags

   NHA

   Expression

   Insect

   Mutation

   The following point mutations were introduced W23…

   Promoter

   Ac5

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCh-695

    Plasmid

    #92263

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 PAM2 mut (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    The following point mutations were introduced: EF…

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMCh-61

    Plasmid

    #92262

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

    Depositor

    Roy Parker, Marina Chekulaeva

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMCh-76

    Plasmid

    #92288

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Roy Parker, Marina Chekulaeva

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 CED (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMCh-646

    Plasmid

    #92289

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 CED 7W7A (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215 with the following point …

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCh-59

    Plasmid

    #92261

    Purpose

    Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Roy Parker, Marina Chekulaeva

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    aa 940-1215

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    p10 and pH

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa

    Plasmid

    #86452

    Purpose

    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs

    Depositor

    Gregor Bucher

    Insert

    UAS-Tc'hsp_p-tGFP-SV40
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pFBD-Btk(PH)Akt1(KD)

    Plasmid

    #86591

    Purpose

    Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFBD-Akt13C(WT)

    Plasmid

    #86576

    Purpose

    Expresses cleavable human full-length Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-OSF-TRIM5-21R(1-300)

    Plasmid

    #79029

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5-21R (1-300)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FoRC.PGm

    Plasmid

    #84586

    Purpose

    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

    Depositor

    Francesca Pignoni

    Article

    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAcUW21-hvmeh1

    Plasmid

    #84212

    Purpose

    baculovirus transfer vector containing hvmeh1 coding sequence from Heliothis virescent

    Depositor

    Bruce Hammock

    Article

    Kamita et al Insect Biochem Mol Biol. 2013 Mar;43(3):

    Insert

    meh1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pAcUW21-CqJHE

    Plasmid

    #84213

    Purpose

    baculovirus transfer vector containing cqjhe coding sequence from Culex quinquefasiatus

    Depositor

    Bruce Hammock

    Article

    Kamita et al PLoS One. 2011;6(12):e28392. doi: 10.137

    Insert

    JHE
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pAcUW21-hovimeh1

    Plasmid

    #84211

    Purpose

    baculovirus transfer vector containing hovimeh1 coding sequence from Homalodisca vitripennis

    Depositor

    Bruce Hammock

    Article

    Kamita et al Arch Insect Biochem Physiol. 2013 Aug;83

    Insert

    meh1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

27. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Use

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UAS-Xbp1spliced

    Plasmid

    #80628

    Purpose

    expression of Xbp1 RB isoform in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    RB isoform

    Promoter

    hsp70

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pSGE_975_Nhe2_EcR-mut

    Plasmid

    #71179

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    975_Nhe2_EcR-mut
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    DSCP Drosophila synthetic core promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSGE_717_S2-1_CGCG-to-DFD

    Plasmid

    #71174

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    717_S2-1_CGCG-to-DFD
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    DSCP Drosophila synthetic core promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge