5,046 results

Showing: 5011 - 5040 of 5046 results

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CJ26 (miR-1003 3'ssAG->GA)

Plasmid

#17765

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Mutation

splice site mutation

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ38 (miR-1006 seed mutant perfect site)

Plasmid

#17771

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1006 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ39 (miR-1003 seed mutant perfect site)

Plasmid

#17772

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1003 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ45 (miR-1003 5' splice site mut)

Plasmid

#17775

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 5' splice mut

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Mutation

5' splice site mut + compsensatory stem

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ50 (cg11094 utr mut)

Plasmid

#17778

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg11094 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ58 (cg6551 utr mut)

Plasmid

#17784

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg6551 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1-Flag pBacPAK8-3

Plasmid

#17322

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
NHR1-Flag pBacPAK8-3

Plasmid

#17323

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pAcUW51-CgE

Plasmid

#13762

Depositor

Pamela Bjorkman

Article

Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

Insert

HSV-1 glycoprotein E

Tags

His

Expression

Insect

Mutation

C-terminal region of the HSV-1 gE (KOS strain ect…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-u(L)Cas9

Plasmid

#127385

Purpose

Expresses Cas9 at low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(L)-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-GFP

Plasmid

#126653

Purpose

Inducible expression of Draper-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Crk-mCherry

Plasmid

#126659

Purpose

Inducible expression of Crk-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT vCrk-GFP

Plasmid

#126661

Purpose

Inducible expression of vCrk truncation in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Crk

Expression

Insect

Mutation

residues 1-182

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Ptp69D-GFP

Plasmid

#126663

Purpose

Inducible expression of Ptp69D-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Ptp69D

Expression

Insect

Mutation

A53S, K105N, and A98T- please see depositor comme…

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Expression

Insect

Mutation

K986N- see depositor comments below

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Shark-mCherry

Plasmid

#126668

Purpose

Inducible expression of Shark-mCherry in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark mCherry

Expression

Insect

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

Plasmid

#177887

Purpose

Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV-4xFRB-SNAP tag

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac(His10-Lckg2a,Y394F,Y505F)

Plasmid

#177894

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F,Y505F

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP1

Plasmid

#176690

Purpose

Expression of Chlamydomonas reinhardtii RSP1 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP1

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP2

Plasmid

#176691

Purpose

Expression of Chlamydomonas reinhardtii RSP2 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP2

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-GST-TEV-RSP3(160-516)

Plasmid

#176692

Purpose

Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP3

Tags

GST

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-HISX6-TEV-RSP4

Plasmid

#176693

Purpose

Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP4

Tags

HISX6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP5

Plasmid

#176694

Purpose

Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP5 (RSP5 Chlamydomonas reinhardtii)

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP6

Plasmid

#176695

Purpose

Expression of Chlamydomonas reinhardtii RSP6 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-RSP7

Plasmid

#176696

Purpose

Expression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP7

Expression

Insect

Mutation

None

Promoter

polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge

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