4,908 results

1. pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

   Plasmid

   #192469

   Purpose

   Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Use

   Tags

   AcGFP1

   Expression

   Insect

   Mutation

   Promoter

   Ac5 promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

   Plasmid

   #192705

   Purpose

   Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728

   Depositor

   Sascha Martens

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16L1
   

   Use

   Tags

   -GFP-TEV-StrepII and 10x-His-TEV

   Expression

   Insect

   Mutation

   F32A-I35A-I36A

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAFMW-Nbr[R272A,K276A, R280A,K284A]

   Plasmid

   #188599

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PRE-Hsp70BbCas9_1.0

   Plasmid

   #190795

   Purpose

   PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   PRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PALI1_PIR_pFB1

   Plasmid

   #191249

   Purpose

   Expresses PALI1 from insect cells

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

   Insert

   LCoR
   

   Use

   Tags

   MBP

   Expression

   Insect

   Mutation

   NA

   Promoter

   Polyhedrin

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MON +T111H

   Plasmid

   #187432

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed threonine at 117 to histidine

   Promoter

   PH and p10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAFMW-Nbr[K216E]

   Plasmid

   #188600

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K216E [AAA=> GAA]

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAFMW-Nbr[C399-625 CM]

   Plasmid

   #188588

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler C-terminal domain, catalytic mutant
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   D435A [GAT=>GCC]; E435A [GAA=>GCC]

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAFMW-Nbr[C399-625]-Gwpep(Mm)

   Plasmid

   #188589

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
   

   Use

   Tags

   FLAG-Myc and GWpep mm

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-Nbr[K276A]

    Plasmid

    #188597

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K276A [AAG=>GCG]

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFMW-Nbr[C399-625]-Gwpep(Dm)

    Plasmid

    #188590

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc and Gwpep dm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAFMW-Gwpep(Dm)-Nbr[C399-625]

    Plasmid

    #188592

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc-Gwpep dm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAFMW-Gwpep(Mm)-Nbr[C399-625]

    Plasmid

    #188591

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
    

    Use

    Tags

    FLAG-Myc-Gwpep mm

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAFMW-Nbr[R272A,K276A]

    Plasmid

    #188596

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAFMW-Nbr[K284A]

    Plasmid

    #188595

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K284A [AAG=> GCG]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAFMW-Nbr[R272A]

    Plasmid

    #188594

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAFMW-Nbr-CM

    Plasmid

    #188585

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler Catalytic mutant
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAFMW-Nbr

    Plasmid

    #188584

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAFMW-Nbr[N1-383]

    Plasmid

    #188586

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler N-terminal domain
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge