4,908 results

1. TEG

   Plasmid

   #187421

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   PH and p10

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FER

   Plasmid

   #187418

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   PH and p10

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CHI

   Plasmid

   #187417

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   PH and p10

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLIB_BSSHI_6xHisTEV-WIPI2d

   Plasmid

   #190863

   Purpose

   Plasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216

   Depositor

   Sascha Martens

   Insert

   WIPI2 (WIPI2 Human)
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   SNP C to G at position 4562 (Val)

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc-sgRNA-Cas9.scr_1

   Plasmid

   #190703

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc-sgRNA-Cas9.scr_3

   Plasmid

   #190705

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 3
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-doraWT_rescue_construct

   Plasmid

   #190608

   Purpose

   Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dora[B]_rescue_construct

   Plasmid

   #190637

   Purpose

   Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-dora[B]_rescue_construct

   Plasmid

   #190638

   Purpose

   Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Changed threonine 295 to methionine

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc-sgRNA-Cas9.dora_1

    Plasmid

    #190700

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-sgRNA-Cas9.dora_2

    Plasmid

    #190701

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc-sgRNA-Cas9.dora_3

    Plasmid

    #190702

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 3
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABR mCherry-Bcd-LEXY

    Plasmid

    #182596

    Purpose

    Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    LEXY and mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABR Bcd-LEXY

    Plasmid

    #182594

    Purpose

    Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bicoid (bcd Fly)
    

    Use

    Tags

    LEXY

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IR25a-T2A-QF2

    Plasmid

    #162522

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC8-Mcl1_deltaTM

    Plasmid

    #187118

    Purpose

    insect cell expression of Mcl1-deltaTM

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    MCL-1 (BCL2 Human)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    delta328-350

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC8-DDIT4_delta8

    Plasmid

    #187117

    Purpose

    insect cell expression of DDIT4-delta1-8

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    DDIT4 (DDIT4 Human)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    delta1-8

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8-DDIT4

    Plasmid

    #187116

    Purpose

    insect cell expression of DDIT4

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    DDIT4 (DDIT4 Human)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge