4,908 results

1. pACEBac1-Q22YU3-2xStrep

   Plasmid

   #170315

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
   

   Use

   Tags

   Strep-Tag II

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mdn1-deltaC

   Plasmid

   #171603

   Purpose

   Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   Mdn1
   

   Use

   Tags

   His6 tag

   Expression

   Insect

   Mutation

   C-terminal deletion; a.a. 1-3911 only

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mdn1-FL

   Plasmid

   #171602

   Purpose

   Expresses full length S. pombe Mdn1 in insect cells.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   Mdn1
   

   Use

   Tags

   His6 tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmBelle_1-298_B

   Plasmid

   #145913

   Purpose

   Insect Expression of DmBelle_1-298

   Depositor

   Elisa Izaurralde

   Insert

   DmBelle_1-298 (bel Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   del 158-160 (GGG)

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1C-Fluc-CG7785_A

   Plasmid

   #145863

   Purpose

   Insect Expression of CG7785-3UTR

   Depositor

   Elisa Izaurralde

   Insert

   CG7785-3UTR (CG7785 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   317bp deletion in 3'UTR region (4172-4273bp)…

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG1b_nsp7 (SARS-CoV-2)

   Plasmid

   #169178

   Purpose

   Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   untagged

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBIG1c_nsp8 (SARS-CoV-2)

   Plasmid

   #169179

   Purpose

   Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   untagged

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

    Plasmid

    #169163

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid

    #171574

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1C-Fluc-nerfin_E

    Plasmid

    #146190

    Purpose

    Insect Expression of nerfin-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    nerfin-3UTR (nerfin-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid

    #146166

    Purpose

    Insect Expression of DmUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF2-siRNAres (Upf2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    3 non silent mutations: L1022P, V1097L and N1213S…

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFL GST-UBXN2A

    Plasmid

    #169016

    Purpose

    Expresses GST-UBXN2A (human) in insect cells

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-U63-tgRNA-Gal80

    Plasmid

    #169030

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP-DmTral-R42EF44A_C

    Plasmid

    #146061

    Purpose

    Insect Expression of DmTral-R42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R42E, F44A. Additionally, two silent mutations A1…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

    Plasmid

    #146039

    Purpose

    Insect Expression of DmTral-FDF-ADA

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-FDF-ADA (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    FDF to ADA (407-409) mutation. Additionally, thre…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid

    #170316

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 C-terminal truncation (Delta-C3)
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Deletion of C-terminal C3-finger

    Promoter

    polyhedrin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge