5,046 results

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pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_UAS>MCS

Plasmid

#194958

Purpose

Drosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Expression

Insect

Mutation

20 x UAS sequences

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBID2_MCS>Gal4

Plasmid

#194961

Purpose

Drosophila Gal4 expression under introduced sequence control, PhiC31 transgenesis

Depositor

Brian McCabe

Article

Ruchti et al bioRxiv 2026.02.02.703225

Type

Empty backbone

Expression

Insect

Mutation

Gal4.2

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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13xLexAop2 IVS sfGFP1-10

Plasmid

#221834

Purpose

Plasmid for generating 13xLexAop2 IVS sfGFP1-10 with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

sfGFP1-10

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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10xUAS-wgSS-HA-V5-LaccID-CD2 (fly optimized, repeat fixed)

Plasmid

#234050

Purpose

expresses LaccID on the surface of fly neurons

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID-CD2

Expression

Insect

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-RBAP46/RBBP7

Plasmid

#109210

Purpose

Encodes human full-length RBBP7 (aka RBAP46) to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length RBBP7 (RBBP7 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-SAP30

Plasmid

#109212

Purpose

Encodes human full-length SAP30 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

Human full-length SAP30 (SAP30 Human)

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-SIN3B-3xFLAG

Plasmid

#109214

Purpose

Encodes human full-length SIN3B with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3B (SIN3B Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID

Plasmid

#240231

Purpose

Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

sfGFP-TurboID

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway Cloning

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-GST-FBXO22

Plasmid

#228367

Purpose

express human FBXO22 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

GST

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK12deltaRS/Cyclin K

Plasmid

#231744

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK12 (CDK12 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Strep(II)(C)-CTR9

Plasmid

#231725

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CTR9 (CTR9 Human)

Tags

3C-Strep(II)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK13/Cyclin K

Plasmid

#231731

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK13 (CDK13 Human)
CCNK (CCNK Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Cyclin K

Plasmid

#231718

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CCNK (CCNK Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Ex

Plasmid

#231106

Purpose

Inducible protein expression in insect cell culture. Stable cell line generation.

Depositor

Michael Jeltsch

Type

Empty backbone

Tags

6xHis and V5

Expression

Insect

Promoter

Drosophila metallothionein promoter, MT

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC (Y530F)

Plasmid

#223743

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Src Kinase (SRC Human)

Tags

GST-TEV

Expression

Insect

Mutation

Y530F constitutively active mutant

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pEW108

Plasmid

#232823

Purpose

Expresses yeast compass complex in insect cells

Depositor

Cynthia Wolberger

Article

Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

Insert

COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)

Tags

His6-3xFLAG, Twinstrep

Expression

Insect

Mutation

Set1(762-1080)

Promoter

polyhedrin promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pZH61 pLIB-8His3C-spt4

Plasmid

#231649

Purpose

express S.cerevisiae spt4 in insect cells

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

spt4 (SPT4 Budding Yeast)

Tags

8His-HRV3C

Expression

Insect

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pZH60 pLIB-spt5

Plasmid

#231648

Purpose

express S.cerevisiae spt5 in insect cells

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

spt5 (SPT5 Budding Yeast)

Expression

Insect

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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78H05-FRT100-STOP-FRT100-p65.AD

Plasmid

#232828

Purpose

Enhancer 78H05 driven p65.AD, gated by Sparse.FRT100

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT100-STOP-FRT100
p65.AD

Expression

Insect

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBP-Sparse.FRT10-p65.AD

Plasmid

#232830

Purpose

Enhancer-free Sparse.FRT10 p65.AD with ccdB for Gateway

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
p65.AD

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBacHTa_LYN

Plasmid

#224244

Purpose

Expression of human LYN Kinase in insect cells (SF9 cells)

Depositor

Andrew Mesecar

Insert

lyn (LYN Human)

Tags

6xHis-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-710)

Plasmid

#228831

Purpose

Insect cell expression of truncated BICD2 (aa 1-710)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 1-710

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Expression

Insect

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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