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5,013 results

Showing: 1561 - 1590 of 5013 results

  5. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  6. ORCO-T2A-QF2

    Plasmid
    #162524
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162524-plasmid-map-sequence-id-354668

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  11. pOPINF Ta-sro1 WWE-PARP

    Plasmid
    #192550
    Purpose
    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)
    Depositor
    Lennart Wirthmueller
    Article
    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d
    Insert
    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    Tags
    His6, 3C protease cleavage site
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available Since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    192550-plasmid-map-sequence-id-378819

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  16. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  22. ULK3 (ULK3A-c002)

    Plasmid
    #110272
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ULK3 (ULK3 Human)
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110272-plasmid-map-sequence-id-212272

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  24. pUAS-kkv-mos::NG

    Plasmid
    #138958
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    8 bp that are in the region that encodes the cata…
    Promoter
    UAS
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    138958-plasmid-map-sequence-id-274779

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Showing: 1561 - 1590 of 5013 results