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pRK5-HA-Sestrin2

Plasmid

#72593

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt11

Plasmid

#42290

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt 11 (Wnt11 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_NES_HaloTag9

Plasmid

#175537

Purpose

Strict cytosolic expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pYFP-NGAT

Plasmid

#11383

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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deltaNp63alpha-FLAG

Plasmid

#26979

Depositor

David Sidransky

Article

Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Insert

ΔNP63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Mutation

dominant negative isoform of p63; aa 1–587

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

Plasmid

#184394

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-EYFP

Plasmid

#128074

Purpose

3rd gen lentiviral plasmid with hUbC-driven EYFP

Depositor

Oskar Laur

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Mutation

no

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRC-CMV-Rev1b

Plasmid

#164443

Purpose

Encodes HIV-1 Rev accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Rev

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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RDX-mCherry

Plasmid

#214476

Purpose

Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

RDX (RDX Human)

Tags

mCherry

Expression

Mammalian

Mutation

Sequence matches cDNA clone MGC:48283 IMAGE:52844…

Promoter

CMV

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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MIG-REST

Plasmid

#91898

Purpose

Expression of mouse REST

Depositor

Julien Sage

Insert

REST (Rest Mouse)

Use

Retroviral

Tags

ires-GFP

Expression

Mammalian

Promoter

MSCV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-3 pMT2

Plasmid

#58727

Purpose

expression of mouse Alpha2 delta-3 in mammalian cells

Depositor

Annette Dolphin

Article

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-3 (Cacna2d3 Mouse)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_AUTS2

Plasmid

#205876

Purpose

Express mEGFP-tagged fusion protein, PAX5_AUTS2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_AUTS2 (PAX5, AUTS2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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hCathepsin L

Plasmid

#11250

Purpose

Mammalian expression of human cathepsin L

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Has Service

Cloning Grade DNA

Insert

cathepsin L (CTSL Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-APEX2-C Cloning Vector

Plasmid

#128146

Purpose

To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

Flag-APEX2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209722

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-hHP1alpha

Plasmid

#181884

Purpose

Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-hHP1alpha (CBX5 Mustard Weed, Human)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh1

Plasmid

#168984

Purpose

Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

Plasmid

#197063

Purpose

Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Inserts

Encapsulin
Adaptor Protein

Use

CRISPR

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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