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qTAG-N-Solo-mStayGold-TUBA1B

Plasmid

#227327

Purpose

Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441C

Plasmid

#25081

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-ALKBH5

Plasmid

#38073

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-ColX

Plasmid

#222305

Purpose

Synchronize the trafficking of CollagenX from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)

Expression

Mammalian

Mutation

signal peptide of COL10A1 removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCLucIPZ

Plasmid

#53222

Purpose

Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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tdTomato-Zyxin-6

Plasmid

#58148

Purpose

Localization: Focal Adhesions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Zyxin (ZYX Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
moxGFP

Plasmid

#68070

Purpose

C-terminal fusion to moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A

Plasmid

#41999

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGs-BFP

Plasmid

#127348

Purpose

Expresses BFP constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Blue Fluorescent Protein

Expression

Mammalian

Promoter

CAGGS Promoter

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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epiCRISPR

Plasmid

#135960

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-eGFP

Plasmid

#193023

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3473

Plasmid

#144949

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Lamin A-mRFP

Plasmid

#124268

Purpose

Wild-type Lamin A, C-terminally tagged with mRFP

Depositor

Eric Schirmer

Insert

LMNA (LMNA Human)

Tags

mRFP

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_Empty

Plasmid

#221480

Purpose

Empty vector control for pLX307 overexpresion experiments

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI

Plasmid

#60421

Purpose

Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI

Tags

Nuclear Export Signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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