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pDONR221_OCA2

Plasmid

#132253

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

OCA2 (OCA2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF2345

Plasmid

#142042

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MITF (MITF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

Plasmid

#100834

Purpose

AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CamKII

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 Flag-mCherry-eEF1A1_IRES-GFP

Plasmid

#198383

Purpose

eEF1A1 fluorescent reporter

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eEF1A1 (EEF1A1 Human)

Use

Lentiviral

Tags

Flag-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mGFP

Plasmid

#190027

Purpose

Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

Depositor

Andrew Tutt

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HP21-TPI-WT-no_introns

Plasmid

#131044

Purpose

Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-Wildtype-no introns (TPI1 Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115

Plasmid

#181996

Purpose

Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115 (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1 H363Y

Plasmid

#1792

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H363Y, deacetylase domain mutation

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC16A4

Plasmid

#131911

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A4 (SLC16A4 Human)

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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CL20_mEGFP_NUP98_HOXD13

Plasmid

#205871

Purpose

Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_HOXD13 (NUP98, HOXD13 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF0548

Plasmid

#143041

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PAX8 (PAX8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMOS010: pLX304-CheRiff-GFP

Plasmid

#62986

Purpose

Lentivirus production of CheRiff channelrhodopsin actuator

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Gateway Cloning and Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP

Plasmid

#89684

Purpose

pCAG promoter driving EGFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-S149A

Plasmid

#135998

Purpose

S149A G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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