78,128 results

1. pHAGE2 GFP-Atl1

   Plasmid

   #86679

   Purpose

   Lentivirus to stably express fluorescent protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   ATL1 (ATL1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH-CAG-AtAFB2.F74A-IRES-puro

   Plasmid

   #216245

   Purpose

   Express AtAFB2.F74A mutant without tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP_CD47_LU

   Plasmid

   #65474

   Purpose

   GFP tagged CD47 with its own long 3'UTR

   Depositor

   Christine Mayr

   Article

   Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

   Inserts

   Signal peptide of CD47
   GFP
   CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
   

   Expression

   Mammalian

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-ACE2-GFP

   Plasmid

   #154962

   Purpose

   pcDNA3.1-ACE2-GFP

   Depositor

   Utpal Pajvani

   Article

   Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

   Insert

   ACE2-GFP (ACE2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FL-fibulin-3-V5-lentiviral-GFP

   Plasmid

   #29703

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Available Since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pBabe puro c-met WT

   Plasmid

   #17493

   Depositor

   Joan Brugge

   Article

   Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

   Insert

   c-Met (Met Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV500 A-FOS

   Plasmid

   #33353

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   FOS (Fos Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative of c-Jun; heterodimerizes and i…

   Promoter

   CMV

   Available Since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-multi-EBFP2-NLS (pRW1447)

   Plasmid

   #216219

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO/GFP-GIGYF2

    Plasmid

    #141189

    Purpose

    Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    GIGYF2 (GIGYF2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVITRO1-102.1F10-IgE/λ

    Plasmid

    #50365

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-CAG-flex-lck-smV5-4x6T

    Plasmid

    #196419

    Purpose

    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet_NES_C1

    Plasmid

    #85060

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCTIP2-N106

    Plasmid

    #66808

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagBFP-Nucleus-7

    Plasmid

    #55265

    Purpose

    Localization: Nuclear Localization, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    mTagBFP-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1-Nluc

    Plasmid

    #180501

    Purpose

    Mammalian expression of Nluc

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-AcGFP1-ORP9-PH

    Plasmid

    #214266

    Purpose

    Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    AcGFP1-ORP9(1-111) (Osbpl9 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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