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pGEL686

Plasmid

#207510

Purpose

ePPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Insert

zCas9 (H840A)-NC-M-MLV RT-deltaRH

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL682

Plasmid

#205591

Purpose

PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL683

Plasmid

#205593

Purpose

Cas9-PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E7

Plasmid

#213470

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd-32aa linker-z…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E8

Plasmid

#213471

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL854

Plasmid

#214254

Purpose

Expression eTd-CBE in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM13451

Plasmid

#219522

Purpose

Transient expression of At BGLU10

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At BGLU10

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM19477

Plasmid

#219521

Purpose

Transient expression of At SAT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At SAT

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM19465

Plasmid

#219520

Purpose

Transient expression of At 79B1

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At UGT79B1

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM13437

Plasmid

#219519

Purpose

Transient expression of At 5MAT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At 5MAT

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM13425

Plasmid

#219518

Purpose

Transient expression of At 5GT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At 5GT

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAGM13414

Plasmid

#219517

Purpose

Transient expression of At 3AT1

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At 3AT1

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM81987

Plasmid

#219516

Purpose

Silencing of Nb RT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb RT

Expression

Plant

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLC045 - pL0_CrGLK (CDS1ns)

Plasmid

#203904

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK (without a stop codon)

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN381 - pTRV2-CHLH

Plasmid

#203886

Purpose

Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CHLH fragment

Use

RNAi

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN378 - pTRV2-CrGLK

Plasmid

#203888

Purpose

Viral silencing plasmid targeting the CrGLK gene from C. roseus.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK fragment

Use

RNAi

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1213 - pL0_p16OMT (pro + 5U)

Plasmid

#203890

Purpose

16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

16OMT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI and/or BsaI cut sites for MoClo …

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUN1243 - pL0_pT3O (pro + 5U)

Plasmid

#203891

Purpose

T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3O promoter and 5'UTR

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge