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pGWB515_35S-HA-(ΔTP)DM3(Hh-0)

Plasmid

#229713

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB414_35S-DM3(Col-0)-HA

Plasmid

#229712

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB414_35S-DM3(Hh-0)-HA

Plasmid

#229711

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pMDC32B-BS-AtMIR390a-A18G-B/c

Plasmid

#246716

Purpose

Cloning amiRNA sequences in AtMIR390a-A18G precursors for in planta expression

Depositor

Alberto Carbonell

Article

Llorens-Gamez et al Plant J. 2026 Jan;125(1):e70665. doi: 10

Insert

Basal stem of Arabidopsis MIR390a precursor with A18G mutation & chloramphenicol-ccdB cassette flanked by two BsaI sites (MIR390a Mustard Weed)

Use

RNAi

Expression

Plant

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-DM3(Hh-0)68-510

Plasmid

#229732

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-67 were deleted, 511-515 were deleted

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ166-sbcB-N

Plasmid

#220305

Purpose

Gateway entry plasmid (attL1& attR5) expressing sbcB exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

sbcB-XTEN linker-zSpCas9

Use

CRISPR; Gateway compatible sbcb-xten linker- zspc…

Expression

Plant

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) T165I

Plasmid

#229726

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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EKv444(pIhcsrI677-Cas9 GG destination vector )

Plasmid

#248344

Purpose

Golden Gate destination vector containing S. pyogenes Cas9 nuclease (with BsaI sites removed). For multicassette gRNA assembly and expression in moss Physcomitrium patens (Physcomitrella).

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pART7

Plasmid

#247985

Purpose

Cloning vector to transiently express an inserted gene in plant cells under the control of the 35S CaMV promoter.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pART27::CLOMELEON

Plasmid

#247988

Purpose

Stable expression of the indicator protein CLOMELEON for monitoring chloride concentrations in the cytoplasm of plant cells.

Depositor

Christoph Plieth

Article

Lorenzen et al Plant J. 2004 May;38(3):539-44. doi: 10.

Insert

CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)

Tags

6xHis

Expression

Plant

Mutation

CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…

Promoter

Cauliflower Mosaic Virus 35S promoter (GenBank AJ…

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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