4,551 results
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Plasmid#223433PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT08
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PEP116E
Plasmid#97402Purposebinary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
TagsPlasma membrane targetExpressionPlantAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH-Ubi-miniSdd7-nCas9-PBE
Plasmid#204856PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1, OsU3, 35SAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P1_AtU626Ter26
Plasmid#191769PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECO200
Plasmid#169765PurposeExpress SpCas9 and Basta resistance geneDepositorTypeEmpty backboneUsePlant binary vectorExpressionPlantPromoter35S, AtU6Available SinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
Tags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only