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pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]

Plasmid

#200327

Purpose

mScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Insert

[DDp | wrmScarlet | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

WBGene00007304, ttr-39

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TU#712

Plasmid

#15280

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzyfp

Use

Tags

Expression

Worm

Mutation

S65G, V68L, S72A, and T203Y

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pMyo-2::oxCerulean-oxVenus::Lgg-1

Plasmid

#68043

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-2::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Use

Tags

mito::GCaMP3

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS544

Plasmid

#188882

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Punc-17b

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159855

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSH87

Plasmid

#127705

Purpose

Pflp-13::gfp1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Use

Tags

Expression

Worm

Mutation

Promoter

flp-13

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBCN44-R4R3

Plasmid

#34919

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pRB1083

Plasmid

#71480

Purpose

Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VRER Cas9 variant

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

eft-3 (eef-1A.1)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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L4043

Plasmid

#1624

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

hsp16-415', hsp16-25', IVSA, SV40NLS, S…

Expression

Worm

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBN303

Plasmid

#86730

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Punc-47::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TU#713

Plasmid

#15281

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czyfp

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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L4271

Plasmid

#1646

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T3p, gfpN, gfpS65S, gfpC, unc2217896, unc223827, …

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pmec-4

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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